Kinomic exploration of temozolomide and radiation resistance in Glioblastoma multiforme xenolines

• Published in: Radiotherapy and oncology 2014-06, Vol.111 (3), p.468-474
• Main Authors: Anderson, Joshua C, Duarte, Christine W, Welaya, Karim, Rohrbach, Timothy D, Bredel, Markus, Yang, Eddy S, Choradia, Nirmal V, Thottassery, Jaideep V, Yancey Gillespie, George, Bonner, James A, Willey, Christopher D
• Format: Article
• Language: English
• Subjects: Animals; Antineoplastic Agents, Alkylating - pharmacology; Array profiling; Brain Neoplasms - drug therapy; Brain Neoplasms - enzymology; Brain Neoplasms - pathology; Brain Neoplasms - radiotherapy; Cell Line, Tumor; Dacarbazine - analogs & derivatives; Dacarbazine - pharmacology; Drug Resistance, Neoplasm; Glioblastoma; Glioblastoma - drug therapy; Glioblastoma - enzymology; Glioblastoma - pathology; Glioblastoma - radiotherapy; Hematology, Oncology and Palliative Medicine; Humans; Kinomics; Mice; Mice, Nude; Protein-Tyrosine Kinases - metabolism; Radiation Tolerance; Temozolomide; Xenograft Model Antitumor Assays; Xenolines
• ISSN: 0167-8140; 1879-0887; 1879-0887
• DOI: 10.1016/j.radonc.2014.04.010

Abstract Background and purpose Glioblastoma multiforme (GBM) represents the most common and deadly primary brain malignancy, particularly due to temozolomide (TMZ) and radiation (RT) resistance. To better understand resistance mechanisms, we examined global kinase activity (kinomic profiling) in both treatment sensitive and resistant human GBM patient-derived xenografts (PDX or “xenolines”). Materials and methods Thirteen orthotopically-implanted xenolines were examined including 8 with known RT sensitivity/resistance, while 5 TMZ resistant xenolines were generated through serial TMZ treatment in vivo . Tumors were harvested, prepared as total protein lysates, and kinomically analyzed on a PamStation®12 high-throughput microarray platform with subsequent upstream kinase prediction and network modeling. Results Kinomic profiles indicated elevated tyrosine kinase activity associated with the radiation resistance phenotype, including FAK and FGFR1. Furthermore, network modeling showed VEGFR1/2 and c-Raf hubs could be involved. Analysis of acquired TMZ resistance revealed more kinomic variability among TMZ resistant tumors. Two of the five tumors displayed significantly altered kinase activity in the TMZ resistant xenolines and network modeling indicated PKC, JAK1, PI3K, CDK2, and VEGFR as potential mediators of this resistance. Conclusion GBM xenolines provide a phenotypic model for GBM drug response and resistance that when paired with kinomic profiling identified targetable pathways to inherent (radiation) or acquired (TMZ) resistance.