Chemical Composition, Leishmanicidal and Cytotoxic Activities of the Essential Oils from Mangifera indica L. var. Rosa and Espada

• Published in: BioMed research international 2014-01, Vol.2014 (2014), p.1-9
• Main Authors: Ramos, Eduardo H. S., Moraes, Marcílio M., Nerys, Laís L. de A., Nascimento, Silene Carneiro do, Militão, Gardenia Carmen Gadelha, Figueiredo, Regina Célia Bressan Queiroz, da Câmara, Cláudio A. G., da Silva, Teresinha Gonçalves
• Format: Article
• Language: English
• Subjects: Apoptosis; Bridged Bicyclo Compounds - chemistry; Bridged Bicyclo Compounds - isolation & purification; Cancer; Cell Proliferation - drug effects; Chemical properties; Cytotoxicity; Essences and essential oils; Fruits; Health aspects; HT29 Cells; Humans; Leishmania - drug effects; Leishmaniasis; Leishmaniasis - drug therapy; Leishmaniasis - pathology; Mangifera - chemistry; Mangifera indica; Mango; Membranes; Monoterpenes - chemistry; Monoterpenes - isolation & purification; Neoplasms - drug therapy; Neoplasms - pathology; Oils & fats; Oils, Volatile - administration & dosage; Oils, Volatile - chemistry; Parasites; Parasitic diseases; Rosa; Terpenes - chemistry; Terpenes - isolation & purification
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2014/734946

The essential oils from Mangifera indica var. Rosa and Espada latex were obtained by hydrodistillation and analyzed using GC-FID and GC-MS. Twenty-seven components were identified. The main compound in the essential oil from M. indica var. Espada (EOMiE) was terpinolene (73.6%). The essential oil of M. indica var. Rosa (EOMiR) was characterized by high amounts of β-pinene (40.7%) and terpinolene (28.3%). In the test for leishmanicidal activity against promastigotes forms of L. amazonensis, EOMiR and EOMiE showed IC50 (72 h) of 39.1 and 23.0 μg/mL, respectively. In macrophages, EOMiR and EOMiE showed CC50 of 142.84 and 158.65 μg/mL, respectively. However, both were more specific to the parasite than macrophages, with values of selectivity index of 6.91 for EOMiE and 3.66 for EOMiR. The essential oils were evaluated for their cytotoxicity against the human tumor cells HEp-2, HT-29, NCI-H292, and HL-60. The EOMiR and EOMiE were most effective against the HL-60, with IC50 values of 12.3 and 3.6 μg/mL, respectively. The results demonstrated that the essential oils of M. indica can destroy L. amazonensis and inhibit tumor cell growth. These findings contribute to the knowledge of the Brazilian biodiversity as a source of potential therapeutic agents.