Multimarker Screening of Oxidative Stress in Aging

• Published in: Oxidative medicine and cellular longevity 2014-01, Vol.2014 (2014), p.1-14
• Main Authors: Pelclová, Daniela, Kuzma, Marek, Mikoška, Miloš, Böhmová, Adéla, Syslová, Kamila, Kačer, Petr
• Format: Article
• Language: English
• Subjects: Aging; Analysis; Biomarkers - metabolism; Czech Republic; Dinoprost - analogs & derivatives; Dinoprost - metabolism; DNA Damage; Guanosine - analogs & derivatives; Guanosine - metabolism; Humans; Medical screening; Mortality; Oxidation-reduction reaction; Oxidative Stress; Proteins - chemistry; Proteins - metabolism; Psychological aspects; Review; Tyrosine - analogs & derivatives; Tyrosine - metabolism
• ISSN: 1942-0900; 1942-0994
• DOI: 10.1155/2014/562860

Aging is a complex process of organism decline in physiological functions. There is no clear theory explaining this phenomenon, but the most accepted one is the oxidative stress theory of aging. Biomarkers of oxidative stress, substances, which are formed during oxidative damage of phospholipids, proteins, and nucleic acids, are present in body fluids of diseased people as well as the healthy ones (in a physiological concentration). 8-iso prostaglandin F2α is the most prominent biomarker of phospholipid oxidative damage, o-tyrosine, 3-chlorotyrosine, and 3-nitrotyrosine are biomarkers of protein oxidative damage, and 8-hydroxy-2′-deoxyguanosine and 8-hydroxyguanosine are biomarkers of oxidative damage of nucleic acids. It is thought that the concentration of biomarkers increases as the age of people increases. However, the concentration of biomarkers in body fluids is very low and, therefore, it is necessary to use a sensitive analytical method. A combination of HPLC and MS was chosen to determine biomarker concentration in three groups of healthy people of a different age (twenty, forty, and sixty years) in order to find a difference among the groups.