The Effect of Babesia divergens Infection on the Spleen of Mongolian Gerbils

Babesiosis is caused by intraerythrocytic protozoan parasites transmitted by ticks and affects a wide range of domestic and wild animals and occasionally humans. The current study aimed to investigate the effect of B. divergens infected erythrocytes on spleen histopathology, cell cycle alteration, a...

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Bibliographic Details
Published in:BioMed research international 2014-01, Vol.2014 (2014), p.1-7
Main Authors: Dkhil, Muhammad A., al-Quraishy, Salih, al-Khalifa, Mohamed S.
Format: Article
Language:English
Subjects:
Animals
Antioxidants
Babesia - metabolism
Babesia divergens
Babesiosis
Babesiosis - blood
Babesiosis - pathology
Catalase - blood
Cell Cycle
Deoxyribonucleic acid
DNA
Enzymes
Erythrocytes
Erythrocytes - metabolism
Erythrocytes - parasitology
Erythrocytes - pathology
Free radicals
Gerbillinae
Gerbils
Glutathione - blood
Histopathology
Infections
Ixodidae
Lipid peroxidation
Lipids
Meriones unguiculatus
Oxidative stress
Parasites
Pathogenesis
Spleen
Spleen - metabolism
Spleen - parasitology
Spleen - pathology
Splenic Diseases - blood
Splenic Diseases - parasitology
Splenic Diseases - pathology
Superoxide Dismutase - blood
Zoology
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Summary:Babesiosis is caused by intraerythrocytic protozoan parasites transmitted by ticks and affects a wide range of domestic and wild animals and occasionally humans. The current study aimed to investigate the effect of B. divergens infected erythrocytes on spleen histopathology, cell cycle alteration, and the presence of oxidative stress. Mongolian gerbils were challenged with 5 × 106  Babesia divergens infected erythrocytes. Parasitemia reached approximately 77% at day 5 postinfection. Infection also induced injury of the spleen. This was evidenced with (i) increases in cellular damage of the spleen, (ii) decrease in antioxidant capacity as indicated by decreased glutathione, catalase, and superoxide dismutase levels, (iii) increased production of malondialdehyde and nitric oxide derived products (nitrite/nitrate), and (iv) increased lactic acid dehydrogenase activity and protein carbonyl content in the spleen. Infection interfered with normal cell cycle of the spleen cells at G0/G1, S, and G2/M phases. On the basis of the above results it can be hypothesized that B. divergens infected erythrocytes could alter the spleen histopathology and cause cell cycle alteration and induce oxidative stress in splenic tissue.
ISSN:2314-6133
2314-6141
DOI:10.1155/2014/483854