Mapping Brain Glucose Uptake with Chemical Exchange-Sensitive Spin-Lock Magnetic Resonance Imaging

Uptake of administered D-glucose (Glc) or 2-deoxy-D-glucose (2DG) has been indirectly mapped through the chemical exchange (CE) between glucose hydroxyl and water protons using CE-dependent saturation transfer (glucoCEST) magnetic resonance imaging (MRI). We propose an alternative technique—on-reson...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cerebral blood flow and metabolism 2014-08, Vol.34 (8), p.1402-1410
Main Authors: Jin, Tao, Mehrens, Hunter, Hendrich, Kristy S, Kim, Seong-Gi
Format: Article
Language:English
Subjects:
Animals
Brain - metabolism
Brain Mapping - methods
Computer Simulation
Deoxyglucose - administration & dosage
Deoxyglucose - chemistry
Deoxyglucose - metabolism
Dose-Response Relationship, Drug
Glucose - administration & dosage
Glucose - chemistry
Glucose - metabolism
Injections, Intravenous
Magnetic Resonance Imaging - methods
Male
Models, Theoretical
Original
Protons
Rats
Rats, Sprague-Dawley
Sensitivity and Specificity
Water - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Uptake of administered D-glucose (Glc) or 2-deoxy-D-glucose (2DG) has been indirectly mapped through the chemical exchange (CE) between glucose hydroxyl and water protons using CE-dependent saturation transfer (glucoCEST) magnetic resonance imaging (MRI). We propose an alternative technique—on-resonance CE-sensitive spin-lock (CESL) MRI—to enhance responses to glucose changes. Phantom data and simulations suggest higher sensitivity for this ‘glucoCESL’ technique (versus glucoCEST) in the intermediate CE regime relevant to glucose. Simulations of CESL signals also show insensitivity to B0-fluctuations. Several findings are apparent from in vivo glucoCESL studies of rat brain at 9.4 Tesla with intravenous injections. First, dose-dependent responses are nearly linearly for 0.25-, 0.5-, and 1-g/kg Glc administration (obtained with 12-second temporal resolution), with changes robustly detected for all doses. Second, responses at a matched dose of 1 g/kg are much larger and persist for a longer duration for 2DG versus Glc administration, and are minimal for mannitol as an osmolality control. And third, with similar increases in steady-state blood glucose levels, glucoCESL responses are ~2.2 times higher for 2DG versus Glc, consistent with their different metabolic properties. Overall, we show that glucoCESL MRI could be a highly sensitive and quantifiable tool for glucose transport and metabolism studies.
ISSN:0271-678X
1559-7016
DOI:10.1038/jcbfm.2014.97