B1-Type Cyclin-Dependent Kinases Are Essential for the Formation of Stomatal Complexes in Arabidopsis thaliana

Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. In yeasts, only one CDK is sufficient to drive cells through the cell cycle, whereas higher eukaryotes developed a family of related CDKs. Curiously, plants contain a unique class of CDKs (B-type CDKs), whose function is still unc...

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Published in:The Plant cell 2004-04, Vol.16 (4), p.945-955
Main Authors: Boudolf, Véronique, Barrôco, Rosa, Janice de Almeida Engler, Verkest, Aurine, Beeckman, Tom, Naudts, Mirande, Inzé, Dirk, De Veylder, Lieven
Format: Article
Language:English
Subjects:
Arabidopsis - cytology
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis thaliana
Base Sequence
Cell Cycle
Cell Differentiation
Cell division
Cell Size
Cotyledons
Cyclin-Dependent Kinases - genetics
Cyclin-Dependent Kinases - metabolism
DNA, Plant - genetics
Epidermal cells
Gene Expression
Genes, Plant
Guard cells
Meristem - cytology
Meristem - metabolism
Mutation
Plant cells
Plants
Plants, Genetically Modified
Promoter Regions, Genetic
Stem cells
Stomata
Transgenic plants
Yeasts
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container_end_page 955
container_issue 4
container_start_page 945
container_title The Plant cell
container_volume 16
creator Boudolf, Véronique
Barrôco, Rosa
Janice de Almeida Engler
Verkest, Aurine
Beeckman, Tom
Naudts, Mirande
Inzé, Dirk
De Veylder, Lieven
description Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. In yeasts, only one CDK is sufficient to drive cells through the cell cycle, whereas higher eukaryotes developed a family of related CDKs. Curiously, plants contain a unique class of CDKs (B-type CDKs), whose function is still unclear. We show that the CDKB1;1 gene of Arabidopsis (Arabidopsis thaliana) is highly expressed in guard cells and stomatal precursor cells of cotyledons, suggesting a prominent role for B-type CDKs in stomatal development. In accordance, transgenic Arabidopsis plants with reduced B-type CDK activity had a decreased stomatal index because of an early block of meristemoid division and inhibition of satellite meristemoid formation. Many aberrant stomatal cells were observed, all of them blocked in the G2 phase of the cell cycle. Although division of stomatal precursors was inhibited, cells still acquired stomatal identity, illustrating that stomatal cell differentiation is independent of cellular and nuclear division.
doi_str_mv 10.1105/tpc.021774
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source JSTOR Archival Journals and Primary Sources Collection; Oxford Journals Online
subjects Arabidopsis - cytology
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis thaliana
Base Sequence
Cell Cycle
Cell Differentiation
Cell division
Cell Size
Cotyledons
Cyclin-Dependent Kinases - genetics
Cyclin-Dependent Kinases - metabolism
DNA, Plant - genetics
Epidermal cells
Gene Expression
Genes, Plant
Guard cells
Meristem - cytology
Meristem - metabolism
Mutation
Plant cells
Plants
Plants, Genetically Modified
Promoter Regions, Genetic
Stem cells
Stomata
Transgenic plants
Yeasts
title B1-Type Cyclin-Dependent Kinases Are Essential for the Formation of Stomatal Complexes in Arabidopsis thaliana
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