RNF4-dependent hybrid SUMO-ubiquitin chains are signals for RAP80 and thereby mediate the recruitment of BRCA1 to sites of DNA damage

The DNA repair function of the breast cancer susceptibility protein BRCA1 depends in part on its interaction with RAP80, which targets BRCA1 to DNA double-strand breaks (DSBs) through recognition of K63-linked polyubiquitin chains. The localization of BRCA1 to DSBs also requires sumoylation. We demo...

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Bibliographic Details
Published in:Science signaling 2012-12, Vol.5 (253), p.ra88-ra88
Main Authors: Guzzo, Catherine M, Berndsen, Christopher E, Zhu, Jianmei, Gupta, Vibhor, Datta, Ajit, Greenberg, Roger A, Wolberger, Cynthia, Matunis, Michael J
Format: Article
Language:English
Subjects:
Amino Acid Motifs
BRCA1 Protein - genetics
BRCA1 Protein - metabolism
Carrier Proteins - genetics
Carrier Proteins - metabolism
Cell Line, Tumor
DNA Breaks, Double-Stranded
DNA Repair - physiology
DNA-Binding Proteins
Histone Chaperones
Humans
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
SUMO-1 Protein - genetics
SUMO-1 Protein - metabolism
Sumoylation
Transcription Factors - genetics
Transcription Factors - metabolism
Ubiquitin-Protein Ligases - genetics
Ubiquitin-Protein Ligases - metabolism
Ubiquitins - genetics
Ubiquitins - metabolism
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Summary:The DNA repair function of the breast cancer susceptibility protein BRCA1 depends in part on its interaction with RAP80, which targets BRCA1 to DNA double-strand breaks (DSBs) through recognition of K63-linked polyubiquitin chains. The localization of BRCA1 to DSBs also requires sumoylation. We demonstrated that, in addition to having ubiquitin-interacting motifs, RAP80 also contains a SUMO-interacting motif (SIM) that is critical for recruitment to DSBs. In combination with the ubiquitin-binding activity of RAP80, this SIM enabled RAP80 to bind with nanomolar affinity to hybrid chains consisting of ubiquitin conjugated to SUMO. Furthermore, RNF4, a SUMO-targeted ubiquitin E3 ligase that synthesizes hybrid SUMO-ubiquitin chains, localized to DSBs and was critical for the recruitment of RAP80 and BRCA1 to sites of DNA damage. Our findings, therefore, connect ubiquitin- and SUMO-dependent DSB recognition, revealing that RNF4-synthesized hybrid SUMO-ubiquitin chains are recognized by RAP80 to promote BRCA1 recruitment and DNA repair.
ISSN:1945-0877
1937-9145
DOI:10.1126/scisignal.2003485