FANCM-associated proteins MHF1 and MHF2, but not the other Fanconi anemia factors, limit meiotic crossovers

Genetic recombination is important for generating diversity and to ensure faithful segregation of chromosomes at meiosis. However, few crossovers (COs) are formed per meiosis despite an excess of DNA double-strand break precursors. This reflects the existence of active mechanisms that limit CO forma...

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Bibliographic Details
Published in:Nucleic acids research 2014-08, Vol.42 (14), p.9087-9095
Main Authors: Girard, Chloe, Crismani, Wayne, Froger, Nicole, Mazel, Julien, Lemhemdi, Afef, Horlow, Christine, Mercier, Raphael
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Arabidopsis Proteins - genetics
Arabidopsis Proteins - physiology
DNA Helicases - genetics
DNA-Binding Proteins - genetics
DNA-Binding Proteins - physiology
Fanconi Anemia Complementation Group Proteins - genetics
Fanconi Anemia Complementation Group Proteins - physiology
Genome Integrity, Repair and
Life Sciences
Meiosis - genetics
Mutation
Recombination, Genetic
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Summary:Genetic recombination is important for generating diversity and to ensure faithful segregation of chromosomes at meiosis. However, few crossovers (COs) are formed per meiosis despite an excess of DNA double-strand break precursors. This reflects the existence of active mechanisms that limit CO formation. We previously showed that AtFANCM is a meiotic anti-CO factor. The same genetic screen now identified AtMHF2 as another player of the same anti-CO pathway. FANCM and MHF2 are both Fanconi Anemia (FA) associated proteins, prompting us to test the other FA genes conserved in Arabidopsis for a role in CO control at meiosis. This revealed that among the FA proteins tested, only FANCM and its two DNA-binding co-factors MHF1 and MHF2 limit CO formation at meiosis.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gku614