Long noncoding RNA associated-competing endogenous RNAs in gastric cancer

• Published in: Scientific reports 2014-08, Vol.4 (1), p.6088, Article 6088
• Main Authors: Xia, Tian, Liao, Qi, Jiang, Xiaoming, Shao, Yongfu, Xiao, Bingxiu, Xi, Yang, Guo, Junming
• Format: Article
• Language: English
• Subjects: 38; 38/61; 38/77; 692/699/67/1504/1829; 692/699/67/69; Algorithms; Carcinoma - genetics; Carcinoma, Papillary; Carcinoma, Squamous Cell - genetics; Cell Line, Tumor; Cell Transformation, Neoplastic - genetics; Chronic lymphocytic leukemia; DNA microarrays; Female; Gastric cancer; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Neoplastic - genetics; Gene regulation; Head & neck cancer; Head and Neck Neoplasms - genetics; Humanities and Social Sciences; Humans; Interleukin 10; Leukemia; Leukemia, Lymphocytic, Chronic, B-Cell - genetics; Lymphatic leukemia; Male; MicroRNAs - genetics; miRNA; multidisciplinary; Oligonucleotide Array Sequence Analysis; Ovarian cancer; Ovarian Neoplasms - genetics; Papillary thyroid carcinoma; Pituitary; Pituitary (anterior); Pituitary Neoplasms - genetics; Post-transcription; Prostate cancer; Prostatic Neoplasms - genetics; PTEN protein; Regulatory sequences; Retinoblastoma Protein - biosynthesis; Retinoblastoma Protein - genetics; RNA Interference; RNA, Long Noncoding - genetics; RNA, Small Interfering; Runx1 protein; Science; siRNA; Squamous cell carcinoma; Squamous Cell Carcinoma of Head and Neck; Stomach Neoplasms - genetics; Thyroid; Thyroid Cancer, Papillary; Thyroid Neoplasms - genetics; Transcription; Tumors
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep06088

Some long noncoding RNAs (lncRNAs) play important roles in the regulation of gene expression by acting as competing endogenous RNAs (ceRNAs). However, the roles of lncRNA associated-ceRNAs in oncogenesis are not fully understood. Here, based on lncRNA microarray data of gastric cancer, bioinformatic algorithm miRcode and microRNA (miRNA) targets database TarBase, we first constructed an lncRNA-miRNA-mRNA network. Then, we confirmed it by data of six types of other cancer including head and neck squamous cell carcinoma, prostate cancer, papillary thyroid carcinoma, pituitary gonadotrope tumors, ovarian cancer and chronic lymphocytic leukemia. The results showed a clear cancer-associated ceRNA network. Eight lncRNAs (AC009499.1, GACAT1, GACAT3, H19, LINC00152, AP000288.2, FER1L4 and RP4-620F22.3) and nine miRNAs (miR-18a-5p, miR-18b-5p, miR-19a-3p, miR-20b-5p, miR-106a-5p, miR-106b-5p, miR-31-5p, miR-139-5p and miR-195-5p) were involved. For instance, through its miRNA response elements (MREs) to compete for miR-106a-5p, lncRNA-FER1L4 regulates the expression of PTEN, RB1, RUNX1, VEGFA, CDKN1A, E2F1, HIPK3, IL-10 and PAK7. Furthermore, cellular experimental results indicated that FER1L4-small interfering RNA (siRNA) simultaneously suppressed FER1L4 and RB1 mRNA level. These results suggest that lncRNAs harbor MREs and play important roles in post-transcriptional regulation in cancer.