Bone morphogenetic protein-9 induces PDLSCs osteogenic differentiation through the ERK and p38 signal pathways

Periodontal ligament stem cells (PDLSCs) with bone morphogenic ability are used to treat diseases such as periodontitis. Their treatment potential is increased when used in combination with proteins that induce osteogenic differentiation. For example, bone morphogenetic protein-9 (BMP9) has been fou...

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Bibliographic Details
Published in:International journal of medical sciences 2014-01, Vol.11 (10), p.1065-1072
Main Authors: Ye, Guo, Li, Conghua, Xiang, Xuerong, Chen, Chu, Zhang, Ruyi, Yang, Xia, Yu, Xuesong, Wang, Jinhua, Wang, Lan, Shi, Qiong, Weng, Yaguang
Format: Article
Language:English
Subjects:
Adolescent
Alkaline Phosphatase - metabolism
Cell Differentiation - drug effects
Cells, Cultured
Child
Core Binding Factor Alpha 1 Subunit - metabolism
Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors
Extracellular Signal-Regulated MAP Kinases - metabolism
Flavonoids - pharmacology
Growth Differentiation Factor 2 - metabolism
Humans
Imidazoles - pharmacology
Immunohistochemistry
Osteocalcin - metabolism
Osteogenesis - drug effects
Osteopontin - metabolism
p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors
p38 Mitogen-Activated Protein Kinases - metabolism
Periodontal Ligament - cytology
Pyridines - pharmacology
Research Paper
Stem Cells - cytology
Stem Cells - drug effects
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Summary:Periodontal ligament stem cells (PDLSCs) with bone morphogenic ability are used to treat diseases such as periodontitis. Their treatment potential is increased when used in combination with proteins that induce osteogenic differentiation. For example, bone morphogenetic protein-9 (BMP9) has been found to have potent osteogenic activity. In the present study, PDLSCs were isolated from human periodontal membrane and infected with recombinant adenoviruses expressing BMP9 (Ad-BMP9). Levels of osteogenic markers such as runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP), osteopontin (OPN), and osteocalcin (OCN) as well as mineralization ability were measured. The results showed that BMP9 promoted bone formation of PDLSCs. In other experiments, SB203580 and PD98059, which are inhibitors of p38 and ERK1/2, respectively, were used to determine if these kinases are involved in the osteogenic differentiation process. The resulting protein expression profiles and osteogenic markers of PDLSCs revealed that the mitogen-activated protein kinase (MAPK) signaling pathway might play an important role in the process of BMP9-induced osteogenic differentiation of PDLSCs.
ISSN:1449-1907
1449-1907
DOI:10.7150/ijms.8473