Bone morphogenetic protein-9 induces PDLSCs osteogenic differentiation through the ERK and p38 signal pathways

• Published in: International journal of medical sciences 2014-01, Vol.11 (10), p.1065-1072
• Main Authors: Ye, Guo, Li, Conghua, Xiang, Xuerong, Chen, Chu, Zhang, Ruyi, Yang, Xia, Yu, Xuesong, Wang, Jinhua, Wang, Lan, Shi, Qiong, Weng, Yaguang
• Format: Article
• Language: English
• Subjects: Adolescent; Alkaline Phosphatase - metabolism; Cell Differentiation - drug effects; Cells, Cultured; Child; Core Binding Factor Alpha 1 Subunit - metabolism; Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors; Extracellular Signal-Regulated MAP Kinases - metabolism; Flavonoids - pharmacology; Growth Differentiation Factor 2 - metabolism; Humans; Imidazoles - pharmacology; Immunohistochemistry; Osteocalcin - metabolism; Osteogenesis - drug effects; Osteopontin - metabolism; p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors; p38 Mitogen-Activated Protein Kinases - metabolism; Periodontal Ligament - cytology; Pyridines - pharmacology; Research Paper; Stem Cells - cytology; Stem Cells - drug effects
• ISSN: 1449-1907; 1449-1907
• DOI: 10.7150/ijms.8473

Periodontal ligament stem cells (PDLSCs) with bone morphogenic ability are used to treat diseases such as periodontitis. Their treatment potential is increased when used in combination with proteins that induce osteogenic differentiation. For example, bone morphogenetic protein-9 (BMP9) has been found to have potent osteogenic activity. In the present study, PDLSCs were isolated from human periodontal membrane and infected with recombinant adenoviruses expressing BMP9 (Ad-BMP9). Levels of osteogenic markers such as runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP), osteopontin (OPN), and osteocalcin (OCN) as well as mineralization ability were measured. The results showed that BMP9 promoted bone formation of PDLSCs. In other experiments, SB203580 and PD98059, which are inhibitors of p38 and ERK1/2, respectively, were used to determine if these kinases are involved in the osteogenic differentiation process. The resulting protein expression profiles and osteogenic markers of PDLSCs revealed that the mitogen-activated protein kinase (MAPK) signaling pathway might play an important role in the process of BMP9-induced osteogenic differentiation of PDLSCs.