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Depletion of functional ribosomal RNA operons in Escherichia coli causes increased expression of the remaining intact copies

The synthesis of ribosomal RNA is a complex and highly regulated process. To study this process, we have used deletion‐insertions to disrupt sequentially from one to four of the seven rRNA (rrn) operons on the Escherichia coli genome. Inactivation of four rrn operons caused a 2.3‐fold increase in th...

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Published in:The EMBO journal 1993-11, Vol.12 (11), p.4305-4315
Main Authors: Condon, C., French, S., Squires, C., Squires, C.L.
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French, S.
Squires, C.
Squires, C.L.
description The synthesis of ribosomal RNA is a complex and highly regulated process. To study this process, we have used deletion‐insertions to disrupt sequentially from one to four of the seven rRNA (rrn) operons on the Escherichia coli genome. Inactivation of four rrn operons caused a 2.3‐fold increase in the expression of a chloramphenicol acetyl transferase reporter gene fused to the tandem promoters of rrnA and a similar increase in the expression of the trp tRNA gene at the end of rrnC. This reflected enhanced expression of the remaining operons to compensate for having only three intact copies. The elevated expression was caused by an increase in both transcription initiation and RNA polymerase elongation rates specifically on rrn operons and occurred in the absence of changes in the intracellular concentration of ppGpp, suggesting that ppGpp is not involved in the regulation of this phenomenon. We discuss these results in relation to the ribosome feedback inhibition model described by Nomura and coworkers.
doi_str_mv 10.1002/j.1460-2075.1993.tb06115.x
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subjects Bacteriology
Base Sequence
Biological and medical sciences
Cloning, Molecular
DNA, Ribosomal - genetics
DNA, Ribosomal - ultrastructure
DNA-Directed RNA Polymerases - metabolism
Drug Resistance, Microbial - genetics
Escherichia coli
Escherichia coli - genetics
Escherichia coli - growth & development
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Bacterial
Guanosine Tetraphosphate - metabolism
Metabolism. Enzymes
Microbiology
Models, Genetic
Molecular Sequence Data
Mutagenesis, Insertional
Operon - genetics
RNA, Ribosomal - biosynthesis
Sequence Deletion
Transcription, Genetic
title Depletion of functional ribosomal RNA operons in Escherichia coli causes increased expression of the remaining intact copies
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