Bone Regeneration by the Combined Use of Tetrapod-Shaped Calcium Phosphate Granules with Basic Fibroblast Growth Factor-Binding Ion Complex Gel in Canine Segmental Radial Defects

The effect of tetrapod-shaped alpha tricalcium phosphate granules (Tetrabones® [TB]) in combination with basic fibroblast growth factor (bFGF)-binding ion complex gel (f-IC gel) on bone defect repair was examined. Bilateral segmental defects 20-mm long were created in the radius of 5 dogs, stabilize...

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Bibliographic Details
Published in:Journal of Veterinary Medical Science 2014, Vol.76(7), pp.955-961
Main Authors: HONNAMI, Muneki, CHOI, Sungjin, LIU, I-li, KAMIMURA, Wataru, TAGUCHI, Tetsushi, HOJO, Hironori, SHIMOHATA, Nobuyuki, OHBA, Shinsuke, KOYAMA, Hiroyuki, NISHIMURA, Ryohei, CHUNG, Ung-il, SASAKI, Nobuo, MOCHIZUKI, Manabu
Format: Article
Language:English
Subjects:
Animals
bone regeneration
Bone Regeneration - drug effects
Bone Regeneration - physiology
Bone Substitutes
calcium phosphate
Calcium Phosphates - chemistry
collagen
Dogs
fibroblast growth factor
Fibroblast Growth Factor 2 - chemistry
Fibroblast Growth Factor 2 - metabolism
Gels
histomorphometry
Implants, Experimental
Male
Osteogenesis - drug effects
Protein Binding
Surgery
Tissue Scaffolds
Wound Healing - drug effects
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Summary:The effect of tetrapod-shaped alpha tricalcium phosphate granules (Tetrabones® [TB]) in combination with basic fibroblast growth factor (bFGF)-binding ion complex gel (f-IC gel) on bone defect repair was examined. Bilateral segmental defects 20-mm long were created in the radius of 5 dogs, stabilized with a plate and screws and implanted with 1 of the following: TB (TB group), TB and bFGF solution (TB/f group), and TB and f-IC gel (TB/f-IC group). Dogs were euthanized 4 weeks after surgery. Radiographs showed well-placed TB granules in the defects and equal osseous callus formation in all the groups. Histomorphometry revealed that the number of vessels and volume of new bone in the TB/f-IC group were significantly higher than those in the other groups. However, no significant differences in neovascularization and new bone formation were observed between the TB/f and TB groups. Furthermore, no significant difference in the lamellar bone volume or rate of mineral apposition was observed among groups. These results suggest that increased bone formation might have been because of the promotion of neovascularization by the f-IC gel. Therefore, the combinatorial method may provide a suitable scaffold for bone regeneration in large segmental long bone defects.
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.14-0027