Gene expression analysis of pancreatic cystic neoplasm in SV40Tag transgenic mice model

• Published in: World journal of gastroenterology : WJG 2007-04, Vol.13 (15), p.2218-2222
• Main Authors: Feng, Jie, Sun, Qiang, Gao, Cheng, Dong, Juan, Wei, Xiao-Luan, Xing, Hua, Li, Hou-Da
• Format: Article
• Language: English
• Subjects: Animals; Antigens, Polyomavirus Transforming - genetics; Antigens, Polyomavirus Transforming - metabolism; Cell Transformation, Neoplastic - genetics; Cystadenocarcinoma - genetics; Cystadenocarcinoma - metabolism; Cystadenoma - genetics; Cystadenoma - metabolism; Disease Models, Animal; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Hedgehog Proteins - genetics; Hedgehog Proteins - physiology; Mice; Mice, Transgenic; Oligonucleotide Array Sequence Analysis; Pancreatic Neoplasms - genetics; Pancreatic Neoplasms - metabolism; Polymerase Chain Reaction; Rapid Communication; Signal Transduction - genetics; Signal Transduction - physiology; SV40标签; Wnt Proteins - genetics; Wnt Proteins - physiology; 基因表达分析; 胰腺囊性肿瘤; 转基因鼠模型
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v13.i15.2218

AIM： To study the gene expression changes in pancreatic cystic neoplasm in SV40Tag transgenic mice model and to provide information about the prevention, clinical diagnosis and therapy of pancreatic cancer. METHODS： Using the pBC-SV40Tag transgenic mice model of pancreatic cystic neoplasm, we studied the gene expression changes by applying high-density microarrays. Validation of part gene expression profiling data was performed using real-time PCR. RESULTS： By using high-density oligonucleotide microarray, of 14113 genes, 453 were increased and 760 decreased in pancreatic cystic neoplasm, including oncogenes, cell-cycle-related genes, signal transduction-related genes, skeleton-related genes and metabolism-related genes. Among these, we confirmed the changes in Igf, Shh and Wnt signal pathways with real-time PCR. The results of real-time PCR showed similar expression changes in gene chip. CONCLUSION： all the altered expression genes are associated with cell cycle, DNA damage and repair, signal pathway, and metabolism. SV40Tag may cooperate with several proteins in promoting tumorigenesis.