Characterization of hepatic progenitors from human fetal liver using CD34 as a hepatic progenitor marker

To enrich putative hepatic progenitors from the developing human fetal liver using CD34 as a marker. Aborted fetuses of 13-20 wk were used for the isolation of liver cells. The cells were labeled with anti CD34; a marker used for isolating progenitor population and the cells were sorted using magnet...

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Bibliographic Details
Published in:World journal of gastroenterology : WJG 2007-04, Vol.13 (16), p.2319-2323
Main Authors: Nyamath, Parveen, Alvi, Ayesha, Habeeb, Aejaz, Khosla, Sanjeev, Khan, Aleem A, Habibullah, C M
Format: Article
Language:English
Subjects:
alpha-Fetoproteins - analysis
alpha-Fetoproteins - metabolism
Antigens, CD34 - analysis
Antigens, CD34 - metabolism
Biomarkers - analysis
Biomarkers - metabolism
Cell Count
Cell Differentiation - physiology
Cell Proliferation
Cell Separation - methods
Cell Survival - physiology
Cells, Cultured
Fetus - cytology
Flow Cytometry
Humans
Liver - cytology
Liver - embryology
Magnetics
Rapid Communication
Stem Cells - cytology
Stem Cells - immunology
Stem Cells - physiology
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Summary:To enrich putative hepatic progenitors from the developing human fetal liver using CD34 as a marker. Aborted fetuses of 13-20 wk were used for the isolation of liver cells. The cells were labeled with anti CD34; a marker used for isolating progenitor population and the cells were sorted using magnetic cell sorting. The positive fractions of cells were assessed for specific hepatic markers. Further, these cells were cultured in vitro for long term investigation. Flow cytometric and immunocytochemical analysis for alphafetoprotein (AFP) showed that the majority of the enriched CD34 positive cells were positive for AFP. Furthermore, these enriched cells proliferated in the long term and maintained hepatic characteristics in in vitro culture. The study shows that aborted human fetal liver is a potential source for isolation of hepatic progenitors for clinical applications. The study also demonstrates that CD34 can be a good marker for the enrichment of progenitor populations.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v13.i16.2319