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Combination of small interfering RNA and lamivudine on inhibition of human B virus replication in HepG2.2.15 cells
To observe the inhibition of hepatitis B virus (HBV) replication and expression by combination of siRNA and lamivudine in HepG2.2.15 cells. Recombinant plasmid psil-HBV was constructed and transfected into HepG2.2.15 cells. The transfected cells were cultured in lamivudine-containing medium (0.05 mi...
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| Published in: | World journal of gastroenterology : WJG 2007-04, Vol.13 (16), p.2324-2327 |
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| container_title | World journal of gastroenterology : WJG |
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| creator | Li, Gui-Qiu Xu, Wei-Zhen Wang, Jing-Xia Deng, Wen-Wei Li, Di Gu, Hong-Xi |
| description | To observe the inhibition of hepatitis B virus (HBV) replication and expression by combination of siRNA and lamivudine in HepG2.2.15 cells.
Recombinant plasmid psil-HBV was constructed and transfected into HepG2.2.15 cells. The transfected cells were cultured in lamivudine-containing medium (0.05 micromol/L) and harvested at 48, 72 and 96 h. The concentration of HBeAg and HBsAg was determined using ELISA. HBV DNA replication was examined by real-time PCR and the level of HBV mRNA was measured by RT-PCR.
In HepG2.2.15 cells treated with combination of siRNA and lamivudine, the secretion of HBeAg and HBsAg into the supernatant was found to be inhibited by 91.80% and 82.40% (2.89+/-0.48 vs 11.73+/-0.38, P |
| doi_str_mv | 10.3748/wjg.v13.i16.2324 |
| format | article |
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Recombinant plasmid psil-HBV was constructed and transfected into HepG2.2.15 cells. The transfected cells were cultured in lamivudine-containing medium (0.05 micromol/L) and harvested at 48, 72 and 96 h. The concentration of HBeAg and HBsAg was determined using ELISA. HBV DNA replication was examined by real-time PCR and the level of HBV mRNA was measured by RT-PCR.
In HepG2.2.15 cells treated with combination of siRNA and lamivudine, the secretion of HBeAg and HBsAg into the supernatant was found to be inhibited by 91.80% and 82.40% (2.89+/-0.48 vs 11.73+/-0.38, P<0.05; 4.59+/-0.57 vs 16.25+/-0.48, P<0.05) at 96 h, respectively; the number of HBV DNA copies within culture medium was also significantly decreased at 96 h (1.04+/-0.26 vs 8.35+/-0.33, P<0.05). Moreover, mRNA concentration in HepG2.2.15 cells treated with combination of siRNA and lamivudine was obviously lower compared to those treated either with siRNA or lamivudine (19.44+/-0.17 vs 33.27+/-0.21 or 79.9+/-0.13, P<0.05).
Combination of siRNA and lamivudine is more effective in inhibiting HBV replication as compared to the single use of siRNA or lamivudine in HepG2.2.15 cells.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v13.i16.2324</identifier><identifier>PMID: 17511031</identifier><language>eng</language><publisher>United States: Department of Microbiology, Harbin Medical University, Harbin 150081,Heilongjiang Province, China%Department of Histology and Embryology,Jiamusi Medical College, Jiamusi University, Jiamusi 154002,Heilongjiang Province, China%Department of Anesthesiology, Second Affiliated Hospital, Jiamusi University, Jiamusi 154002, Heilongjiang Province, China</publisher><subject>Antiviral Agents - pharmacology ; Antiviral Agents - therapeutic use ; Carcinoma, Hepatocellular - pathology ; Carcinoma, Hepatocellular - virology ; Cell Line, Tumor ; DNA Replication - drug effects ; DNA, Viral - metabolism ; Drug Therapy, Combination ; Hepatitis B - drug therapy ; Hepatitis B e Antigens - metabolism ; Hepatitis B Surface Antigens - metabolism ; Hepatitis B virus - genetics ; Hepatitis B virus - physiology ; Humans ; Lamivudine - pharmacology ; Lamivudine - therapeutic use ; Liver Neoplasms - pathology ; Liver Neoplasms - virology ; Rapid Communication ; RNA, Messenger - metabolism ; RNA, Small Interfering - pharmacology ; RNA, Small Interfering - therapeutic use ; RNA, Viral - metabolism ; Transfection ; Virus Replication - drug effects</subject><ispartof>World journal of gastroenterology : WJG, 2007-04, Vol.13 (16), p.2324-2327</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>2007 Baishideng Publishing Group Co., Limited. All rights reserved. 2007</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-c99fda0e1bf4f038f881b5ba44fe7e1777a380b6ab6ef943e2ba97ec620b0a763</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/wjg/wjg.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147141/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147141/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17511031$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Gui-Qiu</creatorcontrib><creatorcontrib>Xu, Wei-Zhen</creatorcontrib><creatorcontrib>Wang, Jing-Xia</creatorcontrib><creatorcontrib>Deng, Wen-Wei</creatorcontrib><creatorcontrib>Li, Di</creatorcontrib><creatorcontrib>Gu, Hong-Xi</creatorcontrib><title>Combination of small interfering RNA and lamivudine on inhibition of human B virus replication in HepG2.2.15 cells</title><title>World journal of gastroenterology : WJG</title><addtitle>World J Gastroenterol</addtitle><description>To observe the inhibition of hepatitis B virus (HBV) replication and expression by combination of siRNA and lamivudine in HepG2.2.15 cells.
Recombinant plasmid psil-HBV was constructed and transfected into HepG2.2.15 cells. The transfected cells were cultured in lamivudine-containing medium (0.05 micromol/L) and harvested at 48, 72 and 96 h. The concentration of HBeAg and HBsAg was determined using ELISA. HBV DNA replication was examined by real-time PCR and the level of HBV mRNA was measured by RT-PCR.
In HepG2.2.15 cells treated with combination of siRNA and lamivudine, the secretion of HBeAg and HBsAg into the supernatant was found to be inhibited by 91.80% and 82.40% (2.89+/-0.48 vs 11.73+/-0.38, P<0.05; 4.59+/-0.57 vs 16.25+/-0.48, P<0.05) at 96 h, respectively; the number of HBV DNA copies within culture medium was also significantly decreased at 96 h (1.04+/-0.26 vs 8.35+/-0.33, P<0.05). Moreover, mRNA concentration in HepG2.2.15 cells treated with combination of siRNA and lamivudine was obviously lower compared to those treated either with siRNA or lamivudine (19.44+/-0.17 vs 33.27+/-0.21 or 79.9+/-0.13, P<0.05).
Combination of siRNA and lamivudine is more effective in inhibiting HBV replication as compared to the single use of siRNA or lamivudine in HepG2.2.15 cells.</description><subject>Antiviral Agents - pharmacology</subject><subject>Antiviral Agents - therapeutic use</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Carcinoma, Hepatocellular - virology</subject><subject>Cell Line, Tumor</subject><subject>DNA Replication - drug effects</subject><subject>DNA, Viral - metabolism</subject><subject>Drug Therapy, Combination</subject><subject>Hepatitis B - drug therapy</subject><subject>Hepatitis B e Antigens - metabolism</subject><subject>Hepatitis B Surface Antigens - metabolism</subject><subject>Hepatitis B virus - genetics</subject><subject>Hepatitis B virus - physiology</subject><subject>Humans</subject><subject>Lamivudine - pharmacology</subject><subject>Lamivudine - therapeutic use</subject><subject>Liver Neoplasms - pathology</subject><subject>Liver Neoplasms - virology</subject><subject>Rapid Communication</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Small Interfering - pharmacology</subject><subject>RNA, Small Interfering - therapeutic use</subject><subject>RNA, Viral - metabolism</subject><subject>Transfection</subject><subject>Virus Replication - drug effects</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNpVkc2P0zAQxS0EYsvCnRPyAXFL8NhOnFyQlgp2kVYgIThbdjpuXSV2sZOu-O9x1fJ1msO89-bjR8hLYLVQsnv7sN_WRxC1h7bmgstHZMU59BXvJHtMVsCYqnrB1RV5lvOeMS5Ew5-SK1ANABOwImkdJ-uDmX0MNDqaJzOO1IcZk8Pkw5Z-_XxDTdjQ0Uz-uGx8QFqkPuy89b9du2Uygb6nR5-WTBMeRj-cI32gd3i45TWvoaEDjmN-Tp44M2Z8canX5PvHD9_Wd9X9l9tP65v7apBczNXQ925jGIJ10jHRua4D21gjpUOFoJQyomO2NbZF10uB3Jpe4dByZplRrbgm7865h8VOuBkwzMmM-pD8ZNJPHY3X_3eC3-ltPGoJUoGEEvD6HPBggjNhq_dxSaGsrMvfefkttIx1RfbmMifFHwvmWU8-ny41AeOStWJNYSJYEbKzcEgx54Tuzy7A9InnKVcXnrrw1CeexfLq3xv-Gi4AxS_EtZ4c</recordid><startdate>20070428</startdate><enddate>20070428</enddate><creator>Li, Gui-Qiu</creator><creator>Xu, Wei-Zhen</creator><creator>Wang, Jing-Xia</creator><creator>Deng, Wen-Wei</creator><creator>Li, Di</creator><creator>Gu, Hong-Xi</creator><general>Department of Microbiology, Harbin Medical University, Harbin 150081,Heilongjiang Province, China%Department of Histology and Embryology,Jiamusi Medical College, Jiamusi University, Jiamusi 154002,Heilongjiang Province, China%Department of Anesthesiology, Second Affiliated Hospital, Jiamusi University, Jiamusi 154002, Heilongjiang Province, China</general><general>Baishideng Publishing Group Co., Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>20070428</creationdate><title>Combination of small interfering RNA and lamivudine on inhibition of human B virus replication in HepG2.2.15 cells</title><author>Li, Gui-Qiu ; Xu, Wei-Zhen ; Wang, Jing-Xia ; Deng, Wen-Wei ; Li, Di ; Gu, Hong-Xi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-c99fda0e1bf4f038f881b5ba44fe7e1777a380b6ab6ef943e2ba97ec620b0a763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Antiviral Agents - pharmacology</topic><topic>Antiviral Agents - therapeutic use</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Carcinoma, Hepatocellular - virology</topic><topic>Cell Line, Tumor</topic><topic>DNA Replication - drug effects</topic><topic>DNA, Viral - metabolism</topic><topic>Drug Therapy, Combination</topic><topic>Hepatitis B - drug therapy</topic><topic>Hepatitis B e Antigens - metabolism</topic><topic>Hepatitis B Surface Antigens - metabolism</topic><topic>Hepatitis B virus - genetics</topic><topic>Hepatitis B virus - physiology</topic><topic>Humans</topic><topic>Lamivudine - pharmacology</topic><topic>Lamivudine - therapeutic use</topic><topic>Liver Neoplasms - pathology</topic><topic>Liver Neoplasms - virology</topic><topic>Rapid Communication</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Small Interfering - pharmacology</topic><topic>RNA, Small Interfering - therapeutic use</topic><topic>RNA, Viral - metabolism</topic><topic>Transfection</topic><topic>Virus Replication - drug effects</topic><toplevel>online_resources</toplevel><creatorcontrib>Li, Gui-Qiu</creatorcontrib><creatorcontrib>Xu, Wei-Zhen</creatorcontrib><creatorcontrib>Wang, Jing-Xia</creatorcontrib><creatorcontrib>Deng, Wen-Wei</creatorcontrib><creatorcontrib>Li, Di</creatorcontrib><creatorcontrib>Gu, Hong-Xi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Gui-Qiu</au><au>Xu, Wei-Zhen</au><au>Wang, Jing-Xia</au><au>Deng, Wen-Wei</au><au>Li, Di</au><au>Gu, Hong-Xi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combination of small interfering RNA and lamivudine on inhibition of human B virus replication in HepG2.2.15 cells</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World J Gastroenterol</addtitle><date>2007-04-28</date><risdate>2007</risdate><volume>13</volume><issue>16</issue><spage>2324</spage><epage>2327</epage><pages>2324-2327</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract>To observe the inhibition of hepatitis B virus (HBV) replication and expression by combination of siRNA and lamivudine in HepG2.2.15 cells.
Recombinant plasmid psil-HBV was constructed and transfected into HepG2.2.15 cells. The transfected cells were cultured in lamivudine-containing medium (0.05 micromol/L) and harvested at 48, 72 and 96 h. The concentration of HBeAg and HBsAg was determined using ELISA. HBV DNA replication was examined by real-time PCR and the level of HBV mRNA was measured by RT-PCR.
In HepG2.2.15 cells treated with combination of siRNA and lamivudine, the secretion of HBeAg and HBsAg into the supernatant was found to be inhibited by 91.80% and 82.40% (2.89+/-0.48 vs 11.73+/-0.38, P<0.05; 4.59+/-0.57 vs 16.25+/-0.48, P<0.05) at 96 h, respectively; the number of HBV DNA copies within culture medium was also significantly decreased at 96 h (1.04+/-0.26 vs 8.35+/-0.33, P<0.05). Moreover, mRNA concentration in HepG2.2.15 cells treated with combination of siRNA and lamivudine was obviously lower compared to those treated either with siRNA or lamivudine (19.44+/-0.17 vs 33.27+/-0.21 or 79.9+/-0.13, P<0.05).
Combination of siRNA and lamivudine is more effective in inhibiting HBV replication as compared to the single use of siRNA or lamivudine in HepG2.2.15 cells.</abstract><cop>United States</cop><pub>Department of Microbiology, Harbin Medical University, Harbin 150081,Heilongjiang Province, China%Department of Histology and Embryology,Jiamusi Medical College, Jiamusi University, Jiamusi 154002,Heilongjiang Province, China%Department of Anesthesiology, Second Affiliated Hospital, Jiamusi University, Jiamusi 154002, Heilongjiang Province, China</pub><pmid>17511031</pmid><doi>10.3748/wjg.v13.i16.2324</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | World journal of gastroenterology : WJG, 2007-04, Vol.13 (16), p.2324-2327 |
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| subjects | Antiviral Agents - pharmacology Antiviral Agents - therapeutic use Carcinoma, Hepatocellular - pathology Carcinoma, Hepatocellular - virology Cell Line, Tumor DNA Replication - drug effects DNA, Viral - metabolism Drug Therapy, Combination Hepatitis B - drug therapy Hepatitis B e Antigens - metabolism Hepatitis B Surface Antigens - metabolism Hepatitis B virus - genetics Hepatitis B virus - physiology Humans Lamivudine - pharmacology Lamivudine - therapeutic use Liver Neoplasms - pathology Liver Neoplasms - virology Rapid Communication RNA, Messenger - metabolism RNA, Small Interfering - pharmacology RNA, Small Interfering - therapeutic use RNA, Viral - metabolism Transfection Virus Replication - drug effects |
| title | Combination of small interfering RNA and lamivudine on inhibition of human B virus replication in HepG2.2.15 cells |
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