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The vacuolar-sorting protein Snf7 is required for export of virulence determinants in members of the Cryptococcus neoformans complex
Fungal pathogenesis requires a number of extracellularly released virulence factors. Recent studies demonstrating that most fungal extracellular molecules lack secretory tags suggest that unconventional secretion mechanisms and fungal virulence are strictly connected. Proteins of the endosomal sorti...
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| Published in: | Scientific reports 2014-09, Vol.4 (1), p.6198-6198, Article 6198 |
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| creator | da C. Godinho, Rodrigo M. Crestani, Juliana Kmetzsch, Lívia de S. Araujo, Glauber Frases, Susana Staats, Charley C. Schrank, Augusto Vainstein, Marilene H. Rodrigues, Marcio L. |
| description | Fungal pathogenesis requires a number of extracellularly released virulence factors. Recent studies demonstrating that most fungal extracellular molecules lack secretory tags suggest that unconventional secretion mechanisms and fungal virulence are strictly connected. Proteins of the endosomal sorting complex required for transport (ESCRT) have been recently associated with polysaccharide export in the yeast-like human pathogen
Cryptococcus neoformans
. Snf7 is a key ESCRT operator required for unconventional secretion in Eukaryotes. In this study we generated
snf7
Δ mutant strains of
C. neoformans
and its sibling species
C. gattii
. Lack of Snf7 resulted in important alterations in polysaccharide secretion, capsular formation and pigmentation. This phenotype culminated with loss of virulence in an intranasal model of murine infection in both species. Our data support the notion that Snf7 expression regulates virulence in
C. neoformans
and
C. gattii
by ablating polysaccharide and melanin traffic. These results are in agreement with the observation that unconventional secretion is essential for cryptococcal pathogenesis and strongly suggest the occurrence of still obscure mechanisms of exportation of non-protein molecules in Eukaryotes. |
| doi_str_mv | 10.1038/srep06198 |
| format | article |
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Cryptococcus neoformans
. Snf7 is a key ESCRT operator required for unconventional secretion in Eukaryotes. In this study we generated
snf7
Δ mutant strains of
C. neoformans
and its sibling species
C. gattii
. Lack of Snf7 resulted in important alterations in polysaccharide secretion, capsular formation and pigmentation. This phenotype culminated with loss of virulence in an intranasal model of murine infection in both species. Our data support the notion that Snf7 expression regulates virulence in
C. neoformans
and
C. gattii
by ablating polysaccharide and melanin traffic. These results are in agreement with the observation that unconventional secretion is essential for cryptococcal pathogenesis and strongly suggest the occurrence of still obscure mechanisms of exportation of non-protein molecules in Eukaryotes.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep06198</identifier><identifier>PMID: 25178636</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>101/28 ; 13/1 ; 14/1 ; 147/135 ; 631/326/193/2538 ; 631/326/88 ; 64/60 ; Animals ; Cryptococcosis - microbiology ; Cryptococcus neoformans - metabolism ; Endosomal Sorting Complexes Required for Transport - metabolism ; Female ; Fungal infections ; Fungal Proteins - metabolism ; Humanities and Social Sciences ; Melanin ; Mice ; Mice, Inbred BALB C ; multidisciplinary ; Pathogenesis ; Pathogens ; Pigmentation ; Polysaccharides - metabolism ; Protein transport ; Protein Transport - physiology ; Science ; Secretion ; Sibling species ; Virulence - physiology ; Virulence factors ; Virulence Factors - metabolism ; Yeasts</subject><ispartof>Scientific reports, 2014-09, Vol.4 (1), p.6198-6198, Article 6198</ispartof><rights>The Author(s) 2014</rights><rights>Copyright Nature Publishing Group Sep 2014</rights><rights>Copyright © 2014, Macmillan Publishers Limited. All rights reserved 2014 Macmillan Publishers Limited. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-feb94412c07d1d2a3281f7cca163e23b7f310402c9ac18b05c66ef7362c75d693</citedby><cites>FETCH-LOGICAL-c438t-feb94412c07d1d2a3281f7cca163e23b7f310402c9ac18b05c66ef7362c75d693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1898056340/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1898056340?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25178636$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>da C. Godinho, Rodrigo M.</creatorcontrib><creatorcontrib>Crestani, Juliana</creatorcontrib><creatorcontrib>Kmetzsch, Lívia</creatorcontrib><creatorcontrib>de S. Araujo, Glauber</creatorcontrib><creatorcontrib>Frases, Susana</creatorcontrib><creatorcontrib>Staats, Charley C.</creatorcontrib><creatorcontrib>Schrank, Augusto</creatorcontrib><creatorcontrib>Vainstein, Marilene H.</creatorcontrib><creatorcontrib>Rodrigues, Marcio L.</creatorcontrib><title>The vacuolar-sorting protein Snf7 is required for export of virulence determinants in members of the Cryptococcus neoformans complex</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Fungal pathogenesis requires a number of extracellularly released virulence factors. Recent studies demonstrating that most fungal extracellular molecules lack secretory tags suggest that unconventional secretion mechanisms and fungal virulence are strictly connected. Proteins of the endosomal sorting complex required for transport (ESCRT) have been recently associated with polysaccharide export in the yeast-like human pathogen
Cryptococcus neoformans
. Snf7 is a key ESCRT operator required for unconventional secretion in Eukaryotes. In this study we generated
snf7
Δ mutant strains of
C. neoformans
and its sibling species
C. gattii
. Lack of Snf7 resulted in important alterations in polysaccharide secretion, capsular formation and pigmentation. This phenotype culminated with loss of virulence in an intranasal model of murine infection in both species. Our data support the notion that Snf7 expression regulates virulence in
C. neoformans
and
C. gattii
by ablating polysaccharide and melanin traffic. These results are in agreement with the observation that unconventional secretion is essential for cryptococcal pathogenesis and strongly suggest the occurrence of still obscure mechanisms of exportation of non-protein molecules in Eukaryotes.</description><subject>101/28</subject><subject>13/1</subject><subject>14/1</subject><subject>147/135</subject><subject>631/326/193/2538</subject><subject>631/326/88</subject><subject>64/60</subject><subject>Animals</subject><subject>Cryptococcosis - microbiology</subject><subject>Cryptococcus neoformans - metabolism</subject><subject>Endosomal Sorting Complexes Required for Transport - metabolism</subject><subject>Female</subject><subject>Fungal infections</subject><subject>Fungal Proteins - metabolism</subject><subject>Humanities and Social Sciences</subject><subject>Melanin</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>multidisciplinary</subject><subject>Pathogenesis</subject><subject>Pathogens</subject><subject>Pigmentation</subject><subject>Polysaccharides - metabolism</subject><subject>Protein transport</subject><subject>Protein Transport - physiology</subject><subject>Science</subject><subject>Secretion</subject><subject>Sibling species</subject><subject>Virulence - physiology</subject><subject>Virulence factors</subject><subject>Virulence Factors - metabolism</subject><subject>Yeasts</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNplkU1r3DAQhk1paUKaQ_9AEfTSFtxqJFu2LoWw9AsCPTQ9C1kebRRsyZHkJbn3h1dh02Xb6iLBPHpmhreqXgJ9D5T3H1LEhQqQ_ZPqlNGmrRln7OnR-6Q6T-mGltMy2YB8Xp2wFrpecHFa_bq6RrLTZg2TjnUKMTu_JUsMGZ0nP7ztiEsk4u3qIo7EhkjwbikYCZbsXFwn9AbJiBnj7Lz2OZHyccZ5wJgeoFwabOL9koMJxqyJeAxFM2ufiAnzMuHdi-qZ1VPC88f7rPr5-dPV5mt9-f3Lt83FZW0a3ufa4iCbBpih3Qgj05z1YDtjNAiOjA-d5UAbyozUBvqBtkYItB0XzHTtKCQ_qz7uvcs6zDga9DnqSS3RzTreq6Cd-rvi3bXahp1qoAWgrAjePApiuF0xZTW7ZHCadFlqTQpaQamUAqCgr_9Bb8IafVlPQS972gre0EK93VMmhlSStIdhgKqHeNUh3sK-Op7-QP4JswDv9kAqJb_FeNTyP9tvhICxtg</recordid><startdate>20140902</startdate><enddate>20140902</enddate><creator>da C. 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Godinho, Rodrigo M. ; Crestani, Juliana ; Kmetzsch, Lívia ; de S. 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Godinho, Rodrigo M.</au><au>Crestani, Juliana</au><au>Kmetzsch, Lívia</au><au>de S. Araujo, Glauber</au><au>Frases, Susana</au><au>Staats, Charley C.</au><au>Schrank, Augusto</au><au>Vainstein, Marilene H.</au><au>Rodrigues, Marcio L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The vacuolar-sorting protein Snf7 is required for export of virulence determinants in members of the Cryptococcus neoformans complex</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2014-09-02</date><risdate>2014</risdate><volume>4</volume><issue>1</issue><spage>6198</spage><epage>6198</epage><pages>6198-6198</pages><artnum>6198</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Fungal pathogenesis requires a number of extracellularly released virulence factors. Recent studies demonstrating that most fungal extracellular molecules lack secretory tags suggest that unconventional secretion mechanisms and fungal virulence are strictly connected. Proteins of the endosomal sorting complex required for transport (ESCRT) have been recently associated with polysaccharide export in the yeast-like human pathogen
Cryptococcus neoformans
. Snf7 is a key ESCRT operator required for unconventional secretion in Eukaryotes. In this study we generated
snf7
Δ mutant strains of
C. neoformans
and its sibling species
C. gattii
. Lack of Snf7 resulted in important alterations in polysaccharide secretion, capsular formation and pigmentation. This phenotype culminated with loss of virulence in an intranasal model of murine infection in both species. Our data support the notion that Snf7 expression regulates virulence in
C. neoformans
and
C. gattii
by ablating polysaccharide and melanin traffic. These results are in agreement with the observation that unconventional secretion is essential for cryptococcal pathogenesis and strongly suggest the occurrence of still obscure mechanisms of exportation of non-protein molecules in Eukaryotes.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>25178636</pmid><doi>10.1038/srep06198</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Scientific reports, 2014-09, Vol.4 (1), p.6198-6198, Article 6198 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4151102 |
| source | Open Access: PubMed Central; Publicly Available Content (ProQuest); Free Full-Text Journals in Chemistry; Springer Nature - nature.com Journals - Fully Open Access |
| subjects | 101/28 13/1 14/1 147/135 631/326/193/2538 631/326/88 64/60 Animals Cryptococcosis - microbiology Cryptococcus neoformans - metabolism Endosomal Sorting Complexes Required for Transport - metabolism Female Fungal infections Fungal Proteins - metabolism Humanities and Social Sciences Melanin Mice Mice, Inbred BALB C multidisciplinary Pathogenesis Pathogens Pigmentation Polysaccharides - metabolism Protein transport Protein Transport - physiology Science Secretion Sibling species Virulence - physiology Virulence factors Virulence Factors - metabolism Yeasts |
| title | The vacuolar-sorting protein Snf7 is required for export of virulence determinants in members of the Cryptococcus neoformans complex |
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