X-ray nanoprobes and diffraction-limited storage rings: opportunities and challenges of fluorescence tomography of biological specimens

X‐ray nanoprobes require coherent illumination to achieve optic‐limited resolution, and so will benefit directly from diffraction‐limited storage rings. Here, the example of high‐resolution X‐ray fluorescence tomography is focused on as one of the most voracious demanders of coherent photons, since...

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Bibliographic Details
Published in:Journal of synchrotron radiation 2014-09, Vol.21 (5), p.1031-1047
Main Authors: de Jonge, Martin D., Ryan, Christopher G., Jacobsen, Chris J.
Format: Article
Language:English
Subjects:
Beams (radiation)
Coherence
Detectors
Diffraction
diffraction-limited storage rings
Diffraction-Limited Storage Rings and New Science Opportunities
Fluorescence
fluorescence tomography
Image Enhancement - instrumentation
Image Enhancement - methods
Microscopy, Fluorescence
Molecular Probe Techniques - instrumentation
Nanoparticles
Nanostructure
nanotomography
Optics and Photonics
Radiation Dosage
scanning X-ray microscopy
Storage rings (particle accelerators)
Synchrotrons
Tomography
X-Ray Diffraction - instrumentation
X-Ray Diffraction - methods
X-ray fluorescence microscopy
X-Ray Microtomography - instrumentation
X-Ray Microtomography - methods
X-rays
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Summary:X‐ray nanoprobes require coherent illumination to achieve optic‐limited resolution, and so will benefit directly from diffraction‐limited storage rings. Here, the example of high‐resolution X‐ray fluorescence tomography is focused on as one of the most voracious demanders of coherent photons, since the detected signal is only a small fraction of the incident flux. Alternative schemes are considered for beam delivery, sample scanning and detectors. One must consider as well the steps before and after the X‐ray experiment: sample preparation and examination conditions, and analysis complexity due to minimum dose requirements and self‐absorption. By understanding the requirements and opportunities for nanoscale fluorescence tomography, one gains insight into the R&D challenges in optics and instrumentation needed to fully exploit the source advances that diffraction‐limited storage rings offer.
ISSN:1600-5775
0909-0495
1600-5775
DOI:10.1107/S160057751401621X