Successful off-label use of the Cepheid Xpert GBS in a late-onset neonatal meningitis by Streptococcus agalactiae

We report the case of a late-onset neonatal meningitis by Streptococcus agalactiae (group B Streptococcus - GBS) that was diagnosed with a latex agglutination assay (on cerebrospinal fluid, CSF), as well as by using, for the first time, Xpert GBS (Cepheid, US) on CSF. Due to empirical antibiotics gi...

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Bibliographic Details
Published in:International journal of clinical and experimental pathology 2014-01, Vol.7 (8), p.5192-5195
Main Authors: Savini, Vincenzo, Marrollo, Roberta, Coclite, Eleonora, Fusilli, Paola, D'Incecco, Carmine, Fazii, Paolo
Format: Article
Language:English
Subjects:
Bacterial Typing Techniques - instrumentation
Bacterial Typing Techniques - methods
Case Report
Humans
Infant
Male
Meningitis, Bacterial - diagnosis
Real-Time Polymerase Chain Reaction - instrumentation
Real-Time Polymerase Chain Reaction - methods
Streptococcal Infections - diagnosis
Streptococcus agalactiae
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Summary:We report the case of a late-onset neonatal meningitis by Streptococcus agalactiae (group B Streptococcus - GBS) that was diagnosed with a latex agglutination assay (on cerebrospinal fluid, CSF), as well as by using, for the first time, Xpert GBS (Cepheid, US) on CSF. Due to empirical antibiotics given before sampling, both CSF and blood culture were negative, so the abovementioned diagnostics was crucial. Moreover, the Xpert GBS assay, performed according to an off-label, modified protocol (the system is designed for GBS-carriage intrapartum screening, based on a completely automated real time-Polymerase Chain Reaction) quickly detected the organism's genome target. Although further investigation on this test's performace on CSF is required, then, we trust it may be a promising, quick and precise diagnostic method for infections in newborns.
ISSN:1936-2625