Molecular imaging of the kinetics of vascular endothelial growth factor gene expression in ischemic myocardium

Angiogenic gene therapy is a promising treatment paradigm for patients with ischemic heart disease. In this study, we used micro-positron emission tomography (microPET) to monitor the transgene expression, function, and effects in a whole-body system. Adenovirus with cytomegalovirus promoter driving...

Full description

Saved in:
Bibliographic Details
Published in:Circulation (New York, N.Y.) N.Y.), 2004-08, Vol.110 (6), p.685-691
Main Authors: Wu, Joseph C, Chen, Ian Y, Wang, Yanling, Tseng, Jeffrey R, Chhabra, Ankush, Salek, Mahdi, Min, Jung-Joon, Fishbein, Michael C, Crystal, Ronald, Gambhir, Sanjiv S
Format: Article
Language:English
Subjects:
Adenovirus
Animals
Cells, Cultured - metabolism
Cytomegalovirus
Feasibility Studies
Female
Fluorodeoxyglucose F18
Gene Expression Profiling - methods
Genes, Reporter
Genetic Therapy
Genetic Vectors - administration & dosage
Genetic Vectors - genetics
Guanine - analogs & derivatives
Heart - diagnostic imaging
Heart Ventricles - diagnostic imaging
Herpes simplex virus 1
Kinetics
Myocardial Contraction
Myocardial Ischemia - diagnostic imaging
Myocardial Ischemia - genetics
Myocardial Ischemia - metabolism
Myocardial Ischemia - therapy
Myocardium - metabolism
Myocytes, Cardiac - metabolism
Neovascularization, Physiologic
Positron-Emission Tomography - methods
Radiopharmaceuticals
Rats
Rats, Sprague-Dawley
Recombinant Fusion Proteins - physiology
Transduction, Genetic
Ultrasonography
Vascular Endothelial Growth Factor A - biosynthesis
Vascular Endothelial Growth Factor A - genetics
Vascular Endothelial Growth Factor A - physiology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Angiogenic gene therapy is a promising treatment paradigm for patients with ischemic heart disease. In this study, we used micro-positron emission tomography (microPET) to monitor the transgene expression, function, and effects in a whole-body system. Adenovirus with cytomegalovirus promoter driving an angiogenic gene (vascular endothelial growth factor [VEGF]) linked to a PET reporter gene (herpes simplex virus type 1 mutant thymidine kinase; Ad-CMV-VEGF121-CMV-HSV1-sr39tk) was used to transfect rat embryonic cardiomyoblasts in vitro. Expression of both genes correlated strongly (r=0.98; P
ISSN:0009-7322
1524-4539
DOI:10.1161/01.cir.000013815302213.22