Up-Regulation of Cluster of Differentiation (CD) 11b Expression on the Surface of Canine Granulocytes with Human Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF)

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a pleiotropic cytokine, sharing a common beta subunit (CDw131) with interleukins 3 and 5. GM-CSF is important for its direct and indirect involvement in host defense. In veterinary medicine, human (h) GM-CSF has been used as a substitute f...

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Bibliographic Details
Published in:Journal of Veterinary Medical Science 2014, Vol.76(8), pp.1173-1176
Main Authors: NAKAGAKI, Kazuhide, NUNOMURA, Yuka, UCHIDA, Kanji, NAKATA, Koh, TAZAWA, Ryushi
Format: Article
Language:English
Subjects:
Animals
canine
CD11b
CD11b Antigen - metabolism
CHO Cells
Cricetinae
Cricetulus
Dogs
Dose-Response Relationship, Drug
Escherichia coli - metabolism
flow cytometry
Gene Expression Regulation - drug effects
Gene Expression Regulation - immunology
granulocyte-macrophage colony-stimulating factor
Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology
Granulocytes - metabolism
Humans
Immunology
median fluorescence intensity
xenostimulation
Yeasts - metabolism
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Summary:Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a pleiotropic cytokine, sharing a common beta subunit (CDw131) with interleukins 3 and 5. GM-CSF is important for its direct and indirect involvement in host defense. In veterinary medicine, human (h) GM-CSF has been used as a substitute for canine GM-CSF to stimulate canine granulocytes and macrophages. In this study, we compared the effects of three distinct hGM-CSFs produced by bacteria, yeasts and Chinese hamster ovary (CHO) cells with those of Escherichia (E) coli-produced canine GM-CSF on the cluster of differentiation 11b (CD11b) expression in canine granulocytes. The median effective dose (ED50) of hGM-CSFs from bacteria, yeasts and CHO cells was 3.09, 4.09 and 4.27 ng/ml, respectively, with no significant difference among three. In contrast, a significant difference was observed between ED50 of canine GM-CSF (0.56 ng/ml) and three hGM-CSFs according to the paired t-test (P
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.14-0056