Three-dimensional reconstructions of the bacteriophage CUS-3 virion reveal a conserved coat protein I-domain but a distinct tailspike receptor-binding domain

Abstract CUS-3 is a short-tailed, dsDNA bacteriophage that infects serotype K1 Escherichia coli . We report icosahedrally averaged and asymmetric, three-dimensional, cryo-electron microscopic reconstructions of the CUS-3 virion. Its coat protein structure adopts the “HK97-fold” shared by other taile...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2014-09, Vol.464, p.55-66
Main Authors: Parent, Kristin N, Tang, Jinghua, Cardone, Giovanni, Gilcrease, Eddie B, Janssen, Mandy E, Olson, Norman H, Casjens, Sherwood R, Baker, Timothy S
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
AMINO ACID SEQUENCE
ASYMMETRY
Bacteriophage
BACTERIOPHAGES
Bacteriophages - chemistry
Bacteriophages - genetics
Bacteriophages - metabolism
Bacteriophages - ultrastructure
Capsid Proteins - chemistry
Capsid Proteins - genetics
Capsid Proteins - metabolism
Capsid Proteins - ultrastructure
Conserved Sequence
Cryo-electron microscopy
Cryoelectron Microscopy
CUS-3
ELECTRON MICROSCOPY
ESCHERICHIA COLI
GENES
Infectious Disease
Models, Molecular
Molecular Sequence Data
MORPHOLOGY
Phylogeny
Protein Binding
PROTEIN STRUCTURE
Protein Structure, Tertiary
RECEPTORS
SYMMETRY
THREE-DIMENSIONAL CALCULATIONS
Virion - chemistry
Virion - genetics
Virion - metabolism
Virion - ultrastructure
Virion structure
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Summary:Abstract CUS-3 is a short-tailed, dsDNA bacteriophage that infects serotype K1 Escherichia coli . We report icosahedrally averaged and asymmetric, three-dimensional, cryo-electron microscopic reconstructions of the CUS-3 virion. Its coat protein structure adopts the “HK97-fold” shared by other tailed phages and is quite similar to that in phages P22 and Sf6 despite only weak amino acid sequence similarity. In addition, these coat proteins share a unique extra external domain (“I-domain”), suggesting that the group of P22-like phages has evolved over a very long time period without acquiring a new coat protein gene from another phage group. On the other hand, the morphology of the CUS-3 tailspike differs significantly from that of P22 or Sf6, but is similar to the tailspike of phage K1F, a member of the extremely distantly related T7 group of phages. We conclude that CUS-3 obtained its tailspike gene from a distantly related phage quite recently.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2014.06.017