Hydrogen sulphide suppresses human atrial fibroblast proliferation and transformation to myofibroblasts

Cardiac fibroblasts are crucial in pathophysiology of the myocardium whereby their aberrant proliferation has significant impact on cardiac function. Hydrogen sulphide (H2S) is a gaseous modulator of potassium channels on cardiomyocytes and has been reported to attenuate cardiac fibrosis. Yet, the m...

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Bibliographic Details
Published in:Journal of cellular and molecular medicine 2013-10, Vol.17 (10), p.1345-1354
Main Authors: Sheng, Jingwei, Shim, Winston, Wei, Heming, Lim, Sze Yun, Liew, Reginald, Lim, Tien Siang, Ong, Boon Hean, Chua, Yeow Leng, Wong, Philip
Format: Article
Language:English
Subjects:
Actin
Angiotensin II
Apoptosis
atrial fibrosis
Base Sequence
Calcium conductance
Calcium ions
Cardiac arrhythmia
Cardiomyocytes
Cell cycle
Cell Differentiation - drug effects
Cell growth
Channel gating
DNA Primers
fibroblast
Fibroblasts
Fibroblasts - drug effects
Fibrosis
Heart
Heart Atria - cytology
Heart Atria - drug effects
Humans
Hydrogen
Hydrogen sulfide
Hydrogen Sulfide - pharmacology
hydrogen sulphide
Immunoglobulins
Kinases
Muscles
Myocardium
Original
Polymerase Chain Reaction
Potassium
potassium channel
Potassium channels
Potassium channels (inwardly-rectifying)
Potassium channels (voltage-gated)
Potassium conductance
Smooth muscle
Software
Transforming growth factor-b1
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Summary:Cardiac fibroblasts are crucial in pathophysiology of the myocardium whereby their aberrant proliferation has significant impact on cardiac function. Hydrogen sulphide (H2S) is a gaseous modulator of potassium channels on cardiomyocytes and has been reported to attenuate cardiac fibrosis. Yet, the mechanism of H2S in modulating proliferation of cardiac fibroblasts remains poorly understood. We hypothesized that H2S inhibits proliferative response of atrial fibroblasts through modulation of potassium channels. Biophysical property of potassium channels in human atrial fibroblasts was examined by whole‐cell patch clamp technique and their cellular proliferation in response to H2S was assessed by BrdU assay. Large conductance Ca2+‐activated K+ current (BKCa), transient outward K+ current (Ito) and inwardly rectifying K+ current (IKir) were found in human atrial fibroblasts. Current density of BKCa (IC50 = 69.4 μM; n = 6), Ito (IC50 = 55.1 μM; n = 6) and IKir (IC50 = 78.9 μM; n = 6) was significantly decreased (P 
ISSN:1582-1838
1582-4934
DOI:10.1111/jcmm.12114