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Early life perfluorooctanesulphonic acid (PFOS) exposure impairs zebrafish organogenesis
•We found PFOS exposure produced uninflated swim bladder and less developed gut.•We examined the histological alterations induced by PFOS.•We examined the detailed structural changes using histology and TEM analysis.•The whole genomic expressions were performed under PFOS window exposure.•Pathways/g...
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| Published in: | Aquatic toxicology 2014-05, Vol.150, p.124-132 |
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| cited_by | cdi_FETCH-LOGICAL-c497t-1cdb578b51d66a38df02fd2f18b09dbd509b9a2682661650dddde4be1be0889d3 |
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| cites | cdi_FETCH-LOGICAL-c497t-1cdb578b51d66a38df02fd2f18b09dbd509b9a2682661650dddde4be1be0889d3 |
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| container_title | Aquatic toxicology |
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| creator | Chen, Jiangfei Tanguay, Robert L. Tal, Tamara L. Gai, Zengxin Ma, Xue Bai, Chenglian Tilton, Susan C. Jin, Daqing Yang, Dongren Huang, Changjiang Dong, Qiaoxiang |
| description | •We found PFOS exposure produced uninflated swim bladder and less developed gut.•We examined the histological alterations induced by PFOS.•We examined the detailed structural changes using histology and TEM analysis.•The whole genomic expressions were performed under PFOS window exposure.•Pathways/genes involved in the PFOS perturbed organogenesis were identified.
As a persistent organic contaminant, perfluorooctanesulphonic acid (PFOS) has been widely detected in the environment, wildlife, and humans. The present study revealed that zebrafish embryos exposed to 16μM PFOS during a sensitive window of 48–96hour post-fertilization (hpf) disrupted larval morphology at 120hpf. Malformed zebrafish larvae were characterized by uninflated swim bladder, less developed gut, and curved spine. Histological and ultrastructural examination of PFOS-exposed larvae showed structural alterations in swim bladder and gut. Whole genome microarray was used to identify the early transcripts dysregulated following exposure to 16μM PFOS at 96hpf. In total, 1278 transcripts were significantly misexpressed (p |
| doi_str_mv | 10.1016/j.aquatox.2014.03.005 |
| format | article |
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As a persistent organic contaminant, perfluorooctanesulphonic acid (PFOS) has been widely detected in the environment, wildlife, and humans. The present study revealed that zebrafish embryos exposed to 16μM PFOS during a sensitive window of 48–96hour post-fertilization (hpf) disrupted larval morphology at 120hpf. Malformed zebrafish larvae were characterized by uninflated swim bladder, less developed gut, and curved spine. Histological and ultrastructural examination of PFOS-exposed larvae showed structural alterations in swim bladder and gut. Whole genome microarray was used to identify the early transcripts dysregulated following exposure to 16μM PFOS at 96hpf. In total, 1278 transcripts were significantly misexpressed (p<0.05) and 211 genes were changed at least two-fold upon PFOS exposure in comparison to the vehicle-exposed control group. A PFOS-induced network of perturbed transcripts relating to swim bladder and gut development revealed that misexpression of genes were involved in organogenesis. Taken together, early life stage exposure to PFOS perturbs various molecular pathways potentially resulting in observed defects in swim bladder and gut development.</description><identifier>ISSN: 0166-445X</identifier><identifier>EISSN: 1879-1514</identifier><identifier>DOI: 10.1016/j.aquatox.2014.03.005</identifier><identifier>PMID: 24667235</identifier><identifier>CODEN: AQTODG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Air Sacs - embryology ; Alkanesulfonic Acids - toxicity ; Animals ; Biological and medical sciences ; Developmental toxicity ; Embryo, Nonmammalian ; Embryology: invertebrates and vertebrates. Teratology ; Environmental Exposure ; Fluorocarbons - toxicity ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental - drug effects ; Gut ; Intestines - embryology ; Organogenesis - drug effects ; Perfluorooctanesulfonic acid ; Swim bladder ; Teratology. Teratogens ; Water Pollutants, Chemical - toxicity ; Zebrafish - embryology ; Zebrafish - genetics ; Zebrafish embryo</subject><ispartof>Aquatic toxicology, 2014-05, Vol.150, p.124-132</ispartof><rights>2014 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c497t-1cdb578b51d66a38df02fd2f18b09dbd509b9a2682661650dddde4be1be0889d3</citedby><cites>FETCH-LOGICAL-c497t-1cdb578b51d66a38df02fd2f18b09dbd509b9a2682661650dddde4be1be0889d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28438190$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24667235$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Jiangfei</creatorcontrib><creatorcontrib>Tanguay, Robert L.</creatorcontrib><creatorcontrib>Tal, Tamara L.</creatorcontrib><creatorcontrib>Gai, Zengxin</creatorcontrib><creatorcontrib>Ma, Xue</creatorcontrib><creatorcontrib>Bai, Chenglian</creatorcontrib><creatorcontrib>Tilton, Susan C.</creatorcontrib><creatorcontrib>Jin, Daqing</creatorcontrib><creatorcontrib>Yang, Dongren</creatorcontrib><creatorcontrib>Huang, Changjiang</creatorcontrib><creatorcontrib>Dong, Qiaoxiang</creatorcontrib><title>Early life perfluorooctanesulphonic acid (PFOS) exposure impairs zebrafish organogenesis</title><title>Aquatic toxicology</title><addtitle>Aquat Toxicol</addtitle><description>•We found PFOS exposure produced uninflated swim bladder and less developed gut.•We examined the histological alterations induced by PFOS.•We examined the detailed structural changes using histology and TEM analysis.•The whole genomic expressions were performed under PFOS window exposure.•Pathways/genes involved in the PFOS perturbed organogenesis were identified.
As a persistent organic contaminant, perfluorooctanesulphonic acid (PFOS) has been widely detected in the environment, wildlife, and humans. The present study revealed that zebrafish embryos exposed to 16μM PFOS during a sensitive window of 48–96hour post-fertilization (hpf) disrupted larval morphology at 120hpf. Malformed zebrafish larvae were characterized by uninflated swim bladder, less developed gut, and curved spine. Histological and ultrastructural examination of PFOS-exposed larvae showed structural alterations in swim bladder and gut. Whole genome microarray was used to identify the early transcripts dysregulated following exposure to 16μM PFOS at 96hpf. In total, 1278 transcripts were significantly misexpressed (p<0.05) and 211 genes were changed at least two-fold upon PFOS exposure in comparison to the vehicle-exposed control group. A PFOS-induced network of perturbed transcripts relating to swim bladder and gut development revealed that misexpression of genes were involved in organogenesis. Taken together, early life stage exposure to PFOS perturbs various molecular pathways potentially resulting in observed defects in swim bladder and gut development.</description><subject>Air Sacs - embryology</subject><subject>Alkanesulfonic Acids - toxicity</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Developmental toxicity</subject><subject>Embryo, Nonmammalian</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Environmental Exposure</subject><subject>Fluorocarbons - toxicity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental - drug effects</subject><subject>Gut</subject><subject>Intestines - embryology</subject><subject>Organogenesis - drug effects</subject><subject>Perfluorooctanesulfonic acid</subject><subject>Swim bladder</subject><subject>Teratology. Teratogens</subject><subject>Water Pollutants, Chemical - toxicity</subject><subject>Zebrafish - embryology</subject><subject>Zebrafish - genetics</subject><subject>Zebrafish embryo</subject><issn>0166-445X</issn><issn>1879-1514</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqFkM1u1DAURi0EotPCI4CyQYJFgp3Yjr2hQlULSJWKBEjdWf65nvEoEwc7qVqevq5mKLDibry45_uudRB6RXBDMOHvt43-ueg53jYtJrTBXYMxe4JWRPSyJozQp2hVOF5Tyq6P0HHOW1ympfI5Omop533bsRW6PtdpuKuG4KGaIPlhiSlGO-sR8jJMmzgGW2kbXPX268XVt3cV3E4xLwmqsJt0SLn6BSZpH_Kmimmtx7iGEg35BXrm9ZDh5eE9QT8uzr-ffa4vrz59Oft4WVsq-7km1hnWC8OI41x3wnncetd6IgyWzjiGpZG65aLlnHCGXRmgBogBLIR03Qn6sO-dFrMDZ2Gckx7UlMJOpzsVdVD_bsawUet4oyhhkveiFLB9gU0x5wT-MUuwelCttuqgWj2oVrhTRXXJvf778GPqt9sCvDkAOls9-KRHG_IfTtBOEIkLd7rnoGi6CZBUtgFGCy4ksLNyMfznK_eawKNd</recordid><startdate>20140501</startdate><enddate>20140501</enddate><creator>Chen, Jiangfei</creator><creator>Tanguay, Robert L.</creator><creator>Tal, Tamara L.</creator><creator>Gai, Zengxin</creator><creator>Ma, Xue</creator><creator>Bai, Chenglian</creator><creator>Tilton, Susan C.</creator><creator>Jin, Daqing</creator><creator>Yang, Dongren</creator><creator>Huang, Changjiang</creator><creator>Dong, Qiaoxiang</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20140501</creationdate><title>Early life perfluorooctanesulphonic acid (PFOS) exposure impairs zebrafish organogenesis</title><author>Chen, Jiangfei ; Tanguay, Robert L. ; Tal, Tamara L. ; Gai, Zengxin ; Ma, Xue ; Bai, Chenglian ; Tilton, Susan C. ; Jin, Daqing ; Yang, Dongren ; Huang, Changjiang ; Dong, Qiaoxiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c497t-1cdb578b51d66a38df02fd2f18b09dbd509b9a2682661650dddde4be1be0889d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Air Sacs - embryology</topic><topic>Alkanesulfonic Acids - toxicity</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Developmental toxicity</topic><topic>Embryo, Nonmammalian</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Environmental Exposure</topic><topic>Fluorocarbons - toxicity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental - drug effects</topic><topic>Gut</topic><topic>Intestines - embryology</topic><topic>Organogenesis - drug effects</topic><topic>Perfluorooctanesulfonic acid</topic><topic>Swim bladder</topic><topic>Teratology. Teratogens</topic><topic>Water Pollutants, Chemical - toxicity</topic><topic>Zebrafish - embryology</topic><topic>Zebrafish - genetics</topic><topic>Zebrafish embryo</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Jiangfei</creatorcontrib><creatorcontrib>Tanguay, Robert L.</creatorcontrib><creatorcontrib>Tal, Tamara L.</creatorcontrib><creatorcontrib>Gai, Zengxin</creatorcontrib><creatorcontrib>Ma, Xue</creatorcontrib><creatorcontrib>Bai, Chenglian</creatorcontrib><creatorcontrib>Tilton, Susan C.</creatorcontrib><creatorcontrib>Jin, Daqing</creatorcontrib><creatorcontrib>Yang, Dongren</creatorcontrib><creatorcontrib>Huang, Changjiang</creatorcontrib><creatorcontrib>Dong, Qiaoxiang</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Aquatic toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Jiangfei</au><au>Tanguay, Robert L.</au><au>Tal, Tamara L.</au><au>Gai, Zengxin</au><au>Ma, Xue</au><au>Bai, Chenglian</au><au>Tilton, Susan C.</au><au>Jin, Daqing</au><au>Yang, Dongren</au><au>Huang, Changjiang</au><au>Dong, Qiaoxiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Early life perfluorooctanesulphonic acid (PFOS) exposure impairs zebrafish organogenesis</atitle><jtitle>Aquatic toxicology</jtitle><addtitle>Aquat Toxicol</addtitle><date>2014-05-01</date><risdate>2014</risdate><volume>150</volume><spage>124</spage><epage>132</epage><pages>124-132</pages><issn>0166-445X</issn><eissn>1879-1514</eissn><coden>AQTODG</coden><abstract>•We found PFOS exposure produced uninflated swim bladder and less developed gut.•We examined the histological alterations induced by PFOS.•We examined the detailed structural changes using histology and TEM analysis.•The whole genomic expressions were performed under PFOS window exposure.•Pathways/genes involved in the PFOS perturbed organogenesis were identified.
As a persistent organic contaminant, perfluorooctanesulphonic acid (PFOS) has been widely detected in the environment, wildlife, and humans. The present study revealed that zebrafish embryos exposed to 16μM PFOS during a sensitive window of 48–96hour post-fertilization (hpf) disrupted larval morphology at 120hpf. Malformed zebrafish larvae were characterized by uninflated swim bladder, less developed gut, and curved spine. Histological and ultrastructural examination of PFOS-exposed larvae showed structural alterations in swim bladder and gut. Whole genome microarray was used to identify the early transcripts dysregulated following exposure to 16μM PFOS at 96hpf. In total, 1278 transcripts were significantly misexpressed (p<0.05) and 211 genes were changed at least two-fold upon PFOS exposure in comparison to the vehicle-exposed control group. A PFOS-induced network of perturbed transcripts relating to swim bladder and gut development revealed that misexpression of genes were involved in organogenesis. Taken together, early life stage exposure to PFOS perturbs various molecular pathways potentially resulting in observed defects in swim bladder and gut development.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>24667235</pmid><doi>10.1016/j.aquatox.2014.03.005</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Aquatic toxicology, 2014-05, Vol.150, p.124-132 |
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| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Air Sacs - embryology Alkanesulfonic Acids - toxicity Animals Biological and medical sciences Developmental toxicity Embryo, Nonmammalian Embryology: invertebrates and vertebrates. Teratology Environmental Exposure Fluorocarbons - toxicity Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental - drug effects Gut Intestines - embryology Organogenesis - drug effects Perfluorooctanesulfonic acid Swim bladder Teratology. Teratogens Water Pollutants, Chemical - toxicity Zebrafish - embryology Zebrafish - genetics Zebrafish embryo |
| title | Early life perfluorooctanesulphonic acid (PFOS) exposure impairs zebrafish organogenesis |
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