Designer Self-Assembling Peptide Nanofiber Scaffolds Containing Link Protein N-Terminal Peptide Induce Chondrogenesis of Rabbit Bone Marrow Stem Cells

• Published in: BioMed research international 2014-01, Vol.2014 (2014), p.1-10
• Main Authors: Liu, Jianxiang, Che, Biao, Yang, Shuhua, Shao, Zengwu, Sun, Caixia, Wang, Baichuan, Wu, Qiang
• Format: Article
• Language: English
• Subjects: Adhesion; Amino Acid Sequence; Animals; Back pain; Biocompatibility; Biosynthesis; Bone marrow; Bone Marrow Cells - cytology; Bone Marrow Cells - drug effects; Bone Marrow Cells - metabolism; Cell Adhesion - drug effects; Cell Death - drug effects; Cell Proliferation - drug effects; Cell Shape - drug effects; Cell Survival - drug effects; Chondrogenesis - drug effects; Collagen; Collagen Type II - metabolism; Extracellular Matrix - metabolism; Extracellular Matrix Proteins - chemistry; Gene Expression Regulation - drug effects; Genotype & phenotype; Hospitals; Hydrogel, Polyethylene Glycol Dimethacrylate - pharmacology; Hydrogels; Laboratory animals; Links; Molecular Sequence Data; Nanofibers - chemistry; Nanostructure; Orthopedics; Peptides; Peptides - chemistry; Peptides - pharmacology; Proteoglycans - chemistry; Rabbits; Rheology - drug effects; Scaffolds; Stem cells; Stem Cells - cytology; Stem Cells - drug effects; Stem Cells - metabolism; Studies; Three dimensional; Tissue engineering; Tissue Scaffolds - chemistry
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2014/421954

Designer self-assembling peptide nanofiber hydrogel scaffolds have been considered as promising biomaterials for tissue engineering because of their excellent biocompatibility and biofunctionality. Our previous studies have shown that a novel designer functionalized self-assembling peptide nanofiber hydrogel scaffold (RLN/RADA16, LN-NS) containing N-terminal peptide sequence of link protein (link N) can promote nucleus pulposus cells (NPCs) adhesion and three-dimensional (3D) migration and stimulate biosynthesis of type II collagen and aggrecan by NPCs in vitro. The present study has extended these investigations to determine the effects of this functionalized LN-NS on bone marrow stem cells (BMSCs), a potential cell source for NP regeneration. Although the functionalized LN-NS cannot promote BMSCs proliferation, it significantly promotes BMSCs adhesion compared with that of the pure RADA16 hydrogel scaffold. Moreover, the functionalized LN-NS remarkably stimulates biosynthesis and deposition of type II collagen and aggrecan. These data demonstrate that the functionalized peptide nanofiber hydrogel scaffold containing link N peptide as a potential matrix substrate will be very useful in the NP tissue regeneration.