The Step Further to Understand the Role of Cytosolic Phospholipase A2 Alpha and Group X Secretory Phospholipase A2 in Allergic Inflammation: Pilot Study

Allergens, viral, and bacterial infections are responsible for asthma exacerbations that occur with progression of airway inflammation. cPLA2α and sPLA2X are responsible for delivery of arachidonic acid for production of eicosanoids—one of the key mediators of airway inflammation. However, cPLA2α an...

Full description

Saved in:
Bibliographic Details
Published in:BioMed research international 2014, Vol.2014 (2014), p.1-9
Main Authors: Kuna, Piotr, Przybek, Monika, Kupryś-Lipińska, Izabela, Sokolowska, Milena, Pniewska, Ewa, Pawliczak, Rafal
Format: Article
Language:English
Subjects:
Adult
Aged
Allergies
Asthma
Asthma - enzymology
Asthma - immunology
Cell Line
Cytokines - immunology
Cytosol - enzymology
Cytosol - immunology
Dermatophagoides pteronyssinus
Drug dosages
Female
Group IV Phospholipases A2 - immunology
Group IV Phospholipases A2 - metabolism
Group X Phospholipases A2 - immunology
Group X Phospholipases A2 - metabolism
Humans
Inflammation - enzymology
Inflammation - immunology
Lung - enzymology
Lung - immunology
Male
Medical research
Middle Aged
Pathogenesis
Phosphorylation
Pilot Projects
Proteins
Rhinitis, Allergic - enzymology
Rhinitis, Allergic - immunology
Rodents
Smooth muscle
Studies
Young Adult
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Allergens, viral, and bacterial infections are responsible for asthma exacerbations that occur with progression of airway inflammation. cPLA2α and sPLA2X are responsible for delivery of arachidonic acid for production of eicosanoids—one of the key mediators of airway inflammation. However, cPLA2α and sPLA2X role in allergic inflammation has not been fully elucidated. The aim of this study was to analyze the influence of rDer p1 and rFel d1 and lipopolysaccharide (LPS) on cPLA2α expression and sPLA2X secretion in PBMC of asthmatics and in A549 cell line. PBMC isolated from 14 subjects, as well as A549 cells, were stimulated with rDer p1, rFel d1, and LPS. Immunoblotting technique was used to study the changes in cPLA2α protein expression and ELISA was used to analyze the release of sPLA2X. PBMC of asthmatics released more sPLA2X than those from healthy controls in the steady state. rDer p1 induced more sPLA2X secretion than cPLA2α protein expression. rFel d1 caused decrease in cPLA2α relative expression in PBMC of asthmatics and in A549 cells. Summarizing, Der p1 and Fel d1 involve phospholipase A2 enzymes in their action. sPLA2X seems to be one of important PLA2 isoform in allergic inflammation, especially caused by house dust mite allergens.
ISSN:2314-6133
2314-6141
DOI:10.1155/2014/670814