Quantitative Analysis of Pork and Chicken Products by Droplet Digital PCR

In this project, a highly precise quantitative method based on the digital polymerase chain reaction (dPCR) technique was developed to determine the weight of pork and chicken in meat products. Real-time quantitative polymerase chain reaction (qPCR) is currently used for quantitative molecular analy...

Full description

Saved in:
Bibliographic Details
Published in:BioMed research international 2014-01, Vol.2014 (2014), p.1-6
Main Authors: Pan, Liangwen, Li, Jian, Yang, Jielin, Lv, Rong, Li, Xiang, Cai, Yicun, He, Yuping
Format: Article
Language:English
Subjects:
Accuracy
Animals
Biomedical research
Chemistry, Analytic
Chickens
Deoxyribonucleic acid
DNA
DNA - analysis
DNA - chemistry
Experiments
Food
Gene expression
Genetic testing
Linear Models
Meat - analysis
Meat - classification
Meat inspection
Meat Products
Meat quality
Methods
Phenols
Polymerase chain reaction
Quantitative
Real-Time Polymerase Chain Reaction - methods
Sensitivity and Specificity
Species Specificity
Swine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c528t-30e8448cc139a55548c67a3fecdb5b2705f0ae0ede84cc7c7458f426095fb6823
cites cdi_FETCH-LOGICAL-c528t-30e8448cc139a55548c67a3fecdb5b2705f0ae0ede84cc7c7458f426095fb6823
container_end_page 6
container_issue 2014
container_start_page 1
container_title BioMed research international
container_volume 2014
creator Pan, Liangwen
Li, Jian
Yang, Jielin
Lv, Rong
Li, Xiang
Cai, Yicun
He, Yuping
description In this project, a highly precise quantitative method based on the digital polymerase chain reaction (dPCR) technique was developed to determine the weight of pork and chicken in meat products. Real-time quantitative polymerase chain reaction (qPCR) is currently used for quantitative molecular analysis of the presence of species-specific DNAs in meat products. However, it is limited in amplification efficiency and relies on standard curves based Ct values, detecting and quantifying low copy number target DNA, as in some complex mixture meat products. By using the dPCR method, we find the relationships between the raw meat weight and DNA weight and between the DNA weight and DNA copy number were both close to linear. This enabled us to establish formulae to calculate the raw meat weight based on the DNA copy number. The accuracy and applicability of this method were tested and verified using samples of pork and chicken powder mixed in known proportions. Quantitative analysis indicated that dPCR is highly precise in quantifying pork and chicken in meat products and therefore has the potential to be used in routine analysis by government regulators and quality control departments of commercial food and feed enterprises.
doi_str_mv 10.1155/2014/810209
format article
fullrecord <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4163444</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A427024541</galeid><sourcerecordid>A427024541</sourcerecordid><originalsourceid>FETCH-LOGICAL-c528t-30e8448cc139a55548c67a3fecdb5b2705f0ae0ede84cc7c7458f426095fb6823</originalsourceid><addsrcrecordid>eNqN0U1vEzEQBmALgWhVeuKOLHFBVKEef61zQYpSPipVIiA4W16vnbjd2MHeLcq_r0NKKFzAF1vy41czHoSeA3kDIMQ5JcDPFRBKpo_QMWXAJxI4PD6cGTtCp6Vck7oUSDKVT9ERFZQzUPwYXX4eTRzCYIZw6_Asmn5bQsHJ40XKN9jEDs9Xwd64iBc5daMdCm63-CKnTe8GfBGW9W2PF_Mvz9ATb_riTu_3E_Tt_buv84-Tq08fLuezq4kVVA0TRpziXFkLbGqEEPUoG8O8s10rWtoQ4YlxxHWVWdvYhgvlOa2FC99KRdkJervP3Yzt2nXWxSGbXm9yWJu81ckE_edNDCu9TLeag2Sc8xrw6j4gp--jK4Neh2Jd35vo0lg0SFAgGCXy31TIWhJIKip9-Re9TmOu__lTESWVAPJbLU3vdIg-1RLtLlTPeG2ecsGhqrO9sjmVkp0_dAdE78aud2PX-7FX_eLhhxzsryFX8HoPViF25kf4vzRXifPmAQYpGs7uADaFuu0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1560868510</pqid></control><display><type>article</type><title>Quantitative Analysis of Pork and Chicken Products by Droplet Digital PCR</title><source>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</source><source>Wiley-Blackwell Titles (Open access)</source><creator>Pan, Liangwen ; Li, Jian ; Yang, Jielin ; Lv, Rong ; Li, Xiang ; Cai, Yicun ; He, Yuping</creator><contributor>Litscher, Gerhard</contributor><creatorcontrib>Pan, Liangwen ; Li, Jian ; Yang, Jielin ; Lv, Rong ; Li, Xiang ; Cai, Yicun ; He, Yuping ; Litscher, Gerhard</creatorcontrib><description>In this project, a highly precise quantitative method based on the digital polymerase chain reaction (dPCR) technique was developed to determine the weight of pork and chicken in meat products. Real-time quantitative polymerase chain reaction (qPCR) is currently used for quantitative molecular analysis of the presence of species-specific DNAs in meat products. However, it is limited in amplification efficiency and relies on standard curves based Ct values, detecting and quantifying low copy number target DNA, as in some complex mixture meat products. By using the dPCR method, we find the relationships between the raw meat weight and DNA weight and between the DNA weight and DNA copy number were both close to linear. This enabled us to establish formulae to calculate the raw meat weight based on the DNA copy number. The accuracy and applicability of this method were tested and verified using samples of pork and chicken powder mixed in known proportions. Quantitative analysis indicated that dPCR is highly precise in quantifying pork and chicken in meat products and therefore has the potential to be used in routine analysis by government regulators and quality control departments of commercial food and feed enterprises.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2014/810209</identifier><identifier>PMID: 25243184</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Accuracy ; Animals ; Biomedical research ; Chemistry, Analytic ; Chickens ; Deoxyribonucleic acid ; DNA ; DNA - analysis ; DNA - chemistry ; Experiments ; Food ; Gene expression ; Genetic testing ; Linear Models ; Meat - analysis ; Meat - classification ; Meat inspection ; Meat Products ; Meat quality ; Methods ; Phenols ; Polymerase chain reaction ; Quantitative ; Real-Time Polymerase Chain Reaction - methods ; Sensitivity and Specificity ; Species Specificity ; Swine</subject><ispartof>BioMed research international, 2014-01, Vol.2014 (2014), p.1-6</ispartof><rights>Copyright © 2014 Yicun Cai et al.</rights><rights>COPYRIGHT 2014 John Wiley &amp; Sons, Inc.</rights><rights>Copyright © 2014 Yicun Cai et al. Yicun Cai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2014 Yicun Cai et al. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c528t-30e8448cc139a55548c67a3fecdb5b2705f0ae0ede84cc7c7458f426095fb6823</citedby><cites>FETCH-LOGICAL-c528t-30e8448cc139a55548c67a3fecdb5b2705f0ae0ede84cc7c7458f426095fb6823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1560868510/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1560868510?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,25753,27924,27925,37012,37013,44590,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25243184$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Litscher, Gerhard</contributor><creatorcontrib>Pan, Liangwen</creatorcontrib><creatorcontrib>Li, Jian</creatorcontrib><creatorcontrib>Yang, Jielin</creatorcontrib><creatorcontrib>Lv, Rong</creatorcontrib><creatorcontrib>Li, Xiang</creatorcontrib><creatorcontrib>Cai, Yicun</creatorcontrib><creatorcontrib>He, Yuping</creatorcontrib><title>Quantitative Analysis of Pork and Chicken Products by Droplet Digital PCR</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>In this project, a highly precise quantitative method based on the digital polymerase chain reaction (dPCR) technique was developed to determine the weight of pork and chicken in meat products. Real-time quantitative polymerase chain reaction (qPCR) is currently used for quantitative molecular analysis of the presence of species-specific DNAs in meat products. However, it is limited in amplification efficiency and relies on standard curves based Ct values, detecting and quantifying low copy number target DNA, as in some complex mixture meat products. By using the dPCR method, we find the relationships between the raw meat weight and DNA weight and between the DNA weight and DNA copy number were both close to linear. This enabled us to establish formulae to calculate the raw meat weight based on the DNA copy number. The accuracy and applicability of this method were tested and verified using samples of pork and chicken powder mixed in known proportions. Quantitative analysis indicated that dPCR is highly precise in quantifying pork and chicken in meat products and therefore has the potential to be used in routine analysis by government regulators and quality control departments of commercial food and feed enterprises.</description><subject>Accuracy</subject><subject>Animals</subject><subject>Biomedical research</subject><subject>Chemistry, Analytic</subject><subject>Chickens</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - analysis</subject><subject>DNA - chemistry</subject><subject>Experiments</subject><subject>Food</subject><subject>Gene expression</subject><subject>Genetic testing</subject><subject>Linear Models</subject><subject>Meat - analysis</subject><subject>Meat - classification</subject><subject>Meat inspection</subject><subject>Meat Products</subject><subject>Meat quality</subject><subject>Methods</subject><subject>Phenols</subject><subject>Polymerase chain reaction</subject><subject>Quantitative</subject><subject>Real-Time Polymerase Chain Reaction - methods</subject><subject>Sensitivity and Specificity</subject><subject>Species Specificity</subject><subject>Swine</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNqN0U1vEzEQBmALgWhVeuKOLHFBVKEef61zQYpSPipVIiA4W16vnbjd2MHeLcq_r0NKKFzAF1vy41czHoSeA3kDIMQ5JcDPFRBKpo_QMWXAJxI4PD6cGTtCp6Vck7oUSDKVT9ERFZQzUPwYXX4eTRzCYIZw6_Asmn5bQsHJ40XKN9jEDs9Xwd64iBc5daMdCm63-CKnTe8GfBGW9W2PF_Mvz9ATb_riTu_3E_Tt_buv84-Tq08fLuezq4kVVA0TRpziXFkLbGqEEPUoG8O8s10rWtoQ4YlxxHWVWdvYhgvlOa2FC99KRdkJervP3Yzt2nXWxSGbXm9yWJu81ckE_edNDCu9TLeag2Sc8xrw6j4gp--jK4Neh2Jd35vo0lg0SFAgGCXy31TIWhJIKip9-Re9TmOu__lTESWVAPJbLU3vdIg-1RLtLlTPeG2ecsGhqrO9sjmVkp0_dAdE78aud2PX-7FX_eLhhxzsryFX8HoPViF25kf4vzRXifPmAQYpGs7uADaFuu0</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Pan, Liangwen</creator><creator>Li, Jian</creator><creator>Yang, Jielin</creator><creator>Lv, Rong</creator><creator>Li, Xiang</creator><creator>Cai, Yicun</creator><creator>He, Yuping</creator><general>Hindawi Publishing Corporation</general><general>John Wiley &amp; Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140101</creationdate><title>Quantitative Analysis of Pork and Chicken Products by Droplet Digital PCR</title><author>Pan, Liangwen ; Li, Jian ; Yang, Jielin ; Lv, Rong ; Li, Xiang ; Cai, Yicun ; He, Yuping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c528t-30e8448cc139a55548c67a3fecdb5b2705f0ae0ede84cc7c7458f426095fb6823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Accuracy</topic><topic>Animals</topic><topic>Biomedical research</topic><topic>Chemistry, Analytic</topic><topic>Chickens</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA - analysis</topic><topic>DNA - chemistry</topic><topic>Experiments</topic><topic>Food</topic><topic>Gene expression</topic><topic>Genetic testing</topic><topic>Linear Models</topic><topic>Meat - analysis</topic><topic>Meat - classification</topic><topic>Meat inspection</topic><topic>Meat Products</topic><topic>Meat quality</topic><topic>Methods</topic><topic>Phenols</topic><topic>Polymerase chain reaction</topic><topic>Quantitative</topic><topic>Real-Time Polymerase Chain Reaction - methods</topic><topic>Sensitivity and Specificity</topic><topic>Species Specificity</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pan, Liangwen</creatorcontrib><creatorcontrib>Li, Jian</creatorcontrib><creatorcontrib>Yang, Jielin</creatorcontrib><creatorcontrib>Lv, Rong</creatorcontrib><creatorcontrib>Li, Xiang</creatorcontrib><creatorcontrib>Cai, Yicun</creatorcontrib><creatorcontrib>He, Yuping</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest_Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Database‎ (1962 - current)</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Middle East &amp; Africa Database</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pan, Liangwen</au><au>Li, Jian</au><au>Yang, Jielin</au><au>Lv, Rong</au><au>Li, Xiang</au><au>Cai, Yicun</au><au>He, Yuping</au><au>Litscher, Gerhard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative Analysis of Pork and Chicken Products by Droplet Digital PCR</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>2014</volume><issue>2014</issue><spage>1</spage><epage>6</epage><pages>1-6</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>In this project, a highly precise quantitative method based on the digital polymerase chain reaction (dPCR) technique was developed to determine the weight of pork and chicken in meat products. Real-time quantitative polymerase chain reaction (qPCR) is currently used for quantitative molecular analysis of the presence of species-specific DNAs in meat products. However, it is limited in amplification efficiency and relies on standard curves based Ct values, detecting and quantifying low copy number target DNA, as in some complex mixture meat products. By using the dPCR method, we find the relationships between the raw meat weight and DNA weight and between the DNA weight and DNA copy number were both close to linear. This enabled us to establish formulae to calculate the raw meat weight based on the DNA copy number. The accuracy and applicability of this method were tested and verified using samples of pork and chicken powder mixed in known proportions. Quantitative analysis indicated that dPCR is highly precise in quantifying pork and chicken in meat products and therefore has the potential to be used in routine analysis by government regulators and quality control departments of commercial food and feed enterprises.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>25243184</pmid><doi>10.1155/2014/810209</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2314-6133
ispartof BioMed research international, 2014-01, Vol.2014 (2014), p.1-6
issn 2314-6133
2314-6141
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4163444
source Publicly Available Content Database (Proquest) (PQ_SDU_P3); Wiley-Blackwell Titles (Open access)
subjects Accuracy
Animals
Biomedical research
Chemistry, Analytic
Chickens
Deoxyribonucleic acid
DNA
DNA - analysis
DNA - chemistry
Experiments
Food
Gene expression
Genetic testing
Linear Models
Meat - analysis
Meat - classification
Meat inspection
Meat Products
Meat quality
Methods
Phenols
Polymerase chain reaction
Quantitative
Real-Time Polymerase Chain Reaction - methods
Sensitivity and Specificity
Species Specificity
Swine
title Quantitative Analysis of Pork and Chicken Products by Droplet Digital PCR
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T07%3A26%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quantitative%20Analysis%20of%20Pork%20and%20Chicken%20Products%20by%20Droplet%20Digital%20PCR&rft.jtitle=BioMed%20research%20international&rft.au=Pan,%20Liangwen&rft.date=2014-01-01&rft.volume=2014&rft.issue=2014&rft.spage=1&rft.epage=6&rft.pages=1-6&rft.issn=2314-6133&rft.eissn=2314-6141&rft_id=info:doi/10.1155/2014/810209&rft_dat=%3Cgale_pubme%3EA427024541%3C/gale_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c528t-30e8448cc139a55548c67a3fecdb5b2705f0ae0ede84cc7c7458f426095fb6823%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1560868510&rft_id=info:pmid/25243184&rft_galeid=A427024541&rfr_iscdi=true