Ezrin silencing remodulates the expression of Phosphoinositide-specific Phospholipase C enzymes in human osteosarcoma cell lines

Ezrin, a protein belonging to the Ezrin, radixin and moesin (ERM) family, was engaged in the metastatic spread of osteosarcoma. The Protein 4.1, Ezrin, radixin, moesin (FERM) domain of Ezrin binds the membrane Phosphatydil inositol (4,5) bisphosphate (PIP2), a crucial molecule belonging to the Phosp...

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Bibliographic Details
Published in:Journal of cell communication and signaling 2014-09, Vol.8 (3), p.219-229
Main Authors: Lo Vasco, V. R., Leopizzi, M., Puggioni, C., Della Rocca, C.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Bone cancer
Cell Biology
Cytoskeleton
Enzymes
Ezrin
Gene silencing
Growth rate
Inositol
Life Sciences
Metastases
Metastasis
Moesin
Osteosarcoma
Phosphatidylinositol 4,5-diphosphate
Phospholipase C
PLC
Protein 4.1
Radixin
Research Article
Signal transduction
Transduction
Tumors
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Summary:Ezrin, a protein belonging to the Ezrin, radixin and moesin (ERM) family, was engaged in the metastatic spread of osteosarcoma. The Protein 4.1, Ezrin, radixin, moesin (FERM) domain of Ezrin binds the membrane Phosphatydil inositol (4,5) bisphosphate (PIP2), a crucial molecule belonging to the Phosphoinositide (PI) signal transduction pathway. The cytoskeleton cross-linker function of Ezrin largely depends on membrane PIP2 levels, and thus upon the activity of related enzymes belonging to the PI-specific phospholipase C (PI-PLC) family. Based on the role of Ezrin in tumour progression and metastasis, we silenced the expression of Vil2 (OMIM *123900), the gene which codifies for Ezrin, in cultured human osteosarcoma 143B and Hs888 cell lines. After Ezrin silencing, the growth rate of both cell lines was significantly reduced and morphogical changes were observed. We also observed moderate variations both of selected PI-PLC enzymes within the cell and of expression of the corresponding PLC genes. In 143B cell line the transcription of PLCB1 decreased, of PLCG2 increased and of PLCE differed in a time-dependent manner. In Hs888, the expression of PLCB1 and of PLCD4 significantly increased, of PLCE moderately increased in a time dependent manner; the expression of PLCG2 was up-regulated. These observations indicate that Ezrin silencing affects the transcription of selected PLC genes, suggesting that Ezrin might influence the expression regulation of PI-PLC enzymes.
ISSN:1873-9601
1873-961X
DOI:10.1007/s12079-014-0235-9