An Air-Well sparging minifermenter system for high-throughput protein production

Over the last few years High-Throughput Protein Production (HTPP) has played a crucial role for functional proteomics. High-quality, high yield and fast recombinant protein production are critical for new HTPP technologies. Escherichia coli is usually the expression system of choice in protein produ...

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Bibliographic Details
Published in:Microbial cell factories 2014-09, Vol.13 (1), p.132-132, Article 132
Main Authors: Deantonio, Cecilia, Sedini, Valentina, Cesaro, Patrizia, Quasso, Fabio, Cotella, Diego, Persichetti, Francesca, Santoro, Claudio, Sblattero, Daniele
Format: Article
Language:English
Subjects:
Air
Air flow
Automation
Bioreactors - microbiology
Biotechnology - instrumentation
Biotechnology - methods
Design
E coli
Escherichia coli
Escherichia coli - growth & development
Escherichia coli - metabolism
Glycerol
High-Throughput Screening Assays - methods
Protein Array Analysis
Proteins
Recombinant Proteins - biosynthesis
Studies
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Summary:Over the last few years High-Throughput Protein Production (HTPP) has played a crucial role for functional proteomics. High-quality, high yield and fast recombinant protein production are critical for new HTPP technologies. Escherichia coli is usually the expression system of choice in protein production thanks to its fast growth, ease of handling and high yields of protein produced. Even though shake-flask cultures are widely used, there is an increasing need for easy to handle, lab scale, high throughput systems. In this article we described a novel minifermenter system suitable for HTPP. The Air-Well minifermenter system is made by a homogeneous air sparging device that includes an air diffusion system, and a stainless steel 96 needle plate integrated with a 96 deep well plate where cultures take place. This system provides aeration to achieve higher optical density growth compared to classical shaking growth without the decrease in pH value and bacterial viability. Moreover the yield of recombinant protein is up to 3-fold higher with a considerable improvement in the amount of full length proteins. High throughput production of hundreds of proteins in parallel can be obtained sparging air in a continuous and controlled manner. The system used is modular and can be easily modified and scaled up to meet the demands for HTPP.
ISSN:1475-2859
1475-2859
DOI:10.1186/s12934-014-0132-1