Engineering Escherichia coli to overproduce aromatic amino acids and derived compounds

The production of aromatic amino acids using fermentation processes with recombinant microorganisms can be an advantageous approach to reach their global demands. In addition, a large array of compounds with alimentary and pharmaceutical applications can potentially be synthesized from intermediates...

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Bibliographic Details
Published in:Microbial cell factories 2014-09, Vol.13 (1), p.126-126, Article 126
Main Authors: Rodriguez, Alberto, Martínez, Juan A, Flores, Noemí, Escalante, Adelfo, Gosset, Guillermo, Bolivar, Francisco
Format: Article
Language:English
Subjects:
Amino acids
Amino Acids, Aromatic - biosynthesis
Amino Acids, Aromatic - chemistry
Aromatic compounds
Biosynthesis
Carbon
Dehydrogenases
E coli
Escherichia coli
Escherichia coli - genetics
Escherichia coli - metabolism
Fermentation
Gene expression
Glucose
Glycerol
Industrial Microbiology
Kinases
Metabolic Engineering
Metabolism
Methods
Physiological aspects
Production processes
Review
Studies
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Summary:The production of aromatic amino acids using fermentation processes with recombinant microorganisms can be an advantageous approach to reach their global demands. In addition, a large array of compounds with alimentary and pharmaceutical applications can potentially be synthesized from intermediates of this metabolic pathway. However, contrary to other amino acids and primary metabolites, the artificial channelling of building blocks from central metabolism towards the aromatic amino acid pathway is complicated to achieve in an efficient manner. The length and complex regulation of this pathway have progressively called for the employment of more integral approaches, promoting the merge of complementary tools and techniques in order to surpass metabolic and regulatory bottlenecks. As a result, relevant insights on the subject have been obtained during the last years, especially with genetically modified strains of Escherichia coli. By combining metabolic engineering strategies with developments in synthetic biology, systems biology and bioprocess engineering, notable advances were achieved regarding the generation, characterization and optimization of E. coli strains for the overproduction of aromatic amino acids, some of their precursors and related compounds. In this paper we review and compare recent successful reports dealing with the modification of metabolic traits to attain these objectives.
ISSN:1475-2859
1475-2859
DOI:10.1186/s12934-014-0126-z