Corolla is a novel protein that contributes to the architecture of the synaptonemal complex of Drosophila

In most organisms the synaptonemal complex (SC) connects paired homologs along their entire length during much of meiotic prophase. To better understand the structure of the SC, we aim to identify its components and to determine how each of these components contributes to SC function. Here, we repor...

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Bibliographic Details
Published in:Genetics (Austin) 2014-09, Vol.198 (1), p.219-228
Main Authors: Collins, Kimberly A, Unruh, Jay R, Slaughter, Brian D, Yu, Zulin, Lake, Cathleen M, Nielsen, Rachel J, Box, Kimberly S, Miller, Danny E, Blumenstiel, Justin P, Perera, Anoja G, Malanowski, Kathryn E, Hawley, R Scott
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Cell division
Chromosomes
Corolla
Drosophila melanogaster
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Female
Genetics
Insects
Investigations
Molecular Sequence Data
Oocytes - metabolism
Protein Binding
Proteins
Synaptonemal Complex - metabolism
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Summary:In most organisms the synaptonemal complex (SC) connects paired homologs along their entire length during much of meiotic prophase. To better understand the structure of the SC, we aim to identify its components and to determine how each of these components contributes to SC function. Here, we report the identification of a novel SC component in Drosophila melanogaster female oocytes, which we have named Corolla. Using structured illumination microscopy, we demonstrate that Corolla is a component of the central region of the SC. Consistent with its localization, we show by yeast two-hybrid analysis that Corolla strongly interacts with Cona, a central element protein, demonstrating the first direct interaction between two inner-synaptonemal complex proteins in Drosophila. These observations help provide a more complete model of SC structure and function in Drosophila females.
ISSN:1943-2631
0016-6731
1943-2631
DOI:10.1534/genetics.114.165290