The Microtubule Plus-End Tracking Protein ARMADILLO-REPEAT KINESIN1 Promotes Microtubule Catastrophe in Arabidopsis

• Published in: The Plant cell 2014-08, Vol.26 (8), p.3372-3386
• Main Authors: Eng, Ryan Christopher, Wasteneys, Geoffrey O.
• Format: Article
• Language: English
• Subjects: Arabidopsis - growth & development; Arabidopsis - metabolism; Arabidopsis - ultrastructure; Arabidopsis Proteins - analysis; Arabidopsis Proteins - metabolism; Arabidopsis Proteins - physiology; Binding Sites; Cell growth; Dinitrobenzenes - pharmacology; Disasters; Epidermal cells; Fluorescence; Kinesin - analysis; Kinesin - metabolism; Kinesin - physiology; Microtubules; Microtubules - metabolism; Microtubules - ultrastructure; Phenotypes; Plant cells; Plant Roots - growth & development; Plant Roots - metabolism; Plant Roots - ultrastructure; Plants; Protein Structure, Tertiary; Proteins; Root hairs; Sulfanilamides - pharmacology
• ISSN: 1040-4651; 1532-298X
• DOI: 10.1105/tpc.114.126789

Microtubule dynamics are critically important for plant cell development. Here, we show that Arabidopsis thaliana ARMADILLOREPEAT KINESIN1 (ARK1) plays a key role in root hair tip growth by promoting microtubule catastrophe events. This destabilizing activity appears to maintain adequate free tubulin concentrations in order to permit rapid microtubule growth, which in turn is correlated with uniform tip growth. Microtubules in ark1-1 root hairs exhibited reduced catastrophe frequency and slower growth velocities, both of which were restored by low concentrations of the microtubule-destabilizing drug oryzalin. An ARK1-GFP (green fluorescent protein) fusion protein expressed under its endogenous promoter localized to growing microtubule plus ends and rescued the ark1-1 root hair phenotype. Transient overexpression of ARK1-RFP (red fluorescent protein) increased microtubule catastrophe frequency. ARK1-fusion protein constructs lacking the N-terminal motor domain still labeled microtubules, suggesting the existence of a second microtubule binding domain at the C terminus of ARK1. ARK1-GFP was broadly expressed in seedlings, but mutant phenotypes were restricted to root hairs, indicating that ARK1's function is redundant in cells other than those forming root hairs.