Novel activators of aquaporin 2 membrane expression for the treatment of nephrogenic diabetes insipidus: less is more. Focus on "High-throughput chemical screening identifies AG-490 as a stimulator of aquaporin 2 membrane expression and urine concentration"

In the present study, Nomura et al report on the development of a novel assay that permits the use of high-throughput screening as a unique means to identify chemical compounds that increase aquaporin 2 (AQP2) expression in the apical plasma membrane. The screening assay was developed from an exocyt...

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Bibliographic Details
Published in:American Journal of Physiology: Cell Physiology 2014-10, Vol.307 (7), p.C595-C596
Main Authors: Sands, Jeff M, Blount, Mitsi A
Format: Article
Language:English
Subjects:
Animals
Aquaporin 2 - drug effects
Cell Membrane - drug effects
Cells
Chemical compounds
Endocytosis
High-Throughput Screening Assays
Kidney - drug effects
Kidney Concentrating Ability - drug effects
Membranes
Protein expression
Tyrphostins - pharmacology
Urological Agents - pharmacology
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Summary:In the present study, Nomura et al report on the development of a novel assay that permits the use of high-throughput screening as a unique means to identify chemical compounds that increase aquaporin 2 (AQP2) expression in the apical plasma membrane. The screening assay was developed from an exocytosis assay that they previously developed and utilizes LLC-PK1 (renal epithelial) cells that express both AQP2 and a soluble secreted yellow fluorescent protein. In the present study, the authors modified this assay for use in 384-well plates, making it possible to perform high-throughput screening.
ISSN:0363-6143
1522-1563
DOI:10.1152/ajpcell.00184.2014