Migration of dendritic cells facilitates systemic dissemination of Burkholderia pseudomallei

Burkholderia pseudomallei, the etiological agent for melioidosis, is an important cause of community-acquired sepsis in northern Australia and northeast Thailand. Due to the rapid dissemination of disease in acute melioidosis, we hypothesized that dendritic cells (DC) could act as a vehicle for diss...

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Bibliographic Details
Published in:Infection and immunity 2014-10, Vol.82 (10), p.4233-4240
Main Authors: Williams, Natasha L, Morris, Jodie L, Rush, Catherine M, Ketheesan, Natkunam
Format: Article
Language:English
Subjects:
Animals
Bacterial Infections
Burkholderia pseudomallei
Burkholderia pseudomallei - immunology
Burkholderia pseudomallei - physiology
Cell Movement
Dendritic Cells - immunology
Dendritic Cells - microbiology
Disease Models, Animal
Endocytosis
Lung - microbiology
Lymphoid Tissue - microbiology
Melioidosis - immunology
Melioidosis - microbiology
Melioidosis - pathology
Mice
Mice, Inbred C57BL
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Summary:Burkholderia pseudomallei, the etiological agent for melioidosis, is an important cause of community-acquired sepsis in northern Australia and northeast Thailand. Due to the rapid dissemination of disease in acute melioidosis, we hypothesized that dendritic cells (DC) could act as a vehicle for dissemination of B. pseudomallei. Therefore, this study investigated the effect of B. pseudomallei infection on DC migration capacity and whether migration of DC enabled transportation of B. pseudomallei from the site of infection. B. pseudomallei stimulated significantly increased migration of bone marrow-derived DC (BMDC), both in vitro and in vivo, compared to uninfected BMDC. Furthermore, migration of BMDC enabled significantly increased in vitro trafficking of B. pseudomallei and in vivo dissemination of B. pseudomallei to secondary lymphoid organs and lungs of C57BL/6 mice. DC within the footpad infection site of C57BL/6 mice also internalized B. pseudomallei and facilitated dissemination. Although DC have previously been shown to kill intracellular B. pseudomallei in vitro, the findings of this study demonstrate that B. pseudomallei-infected DC facilitate the systemic spread of this pathogen.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.01880-14