Standing Surface Acoustic Wave Based Cell Coculture

Precise reconstruction of heterotypic cell–cell interactions in vitro requires the coculture of different cell types in a highly controlled manner. In this article, we report a standing surface acoustic wave (SSAW)-based cell coculture platform. In our approach, different types of cells are patterne...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2014-10, Vol.86 (19), p.9853-9859
Main Authors: Li, Sixing, Guo, Feng, Chen, Yuchao, Ding, Xiaoyun, Li, Peng, Wang, Lin, Cameron, Craig E, Huang, Tony Jun
Format: Article
Language:English
Subjects:
Acoustics
Analytical chemistry
Cancer
Cell adhesion & migration
Cell Communication - physiology
Cell Movement
Cellular
Coculture Techniques
Endothelial cells
Endothelial Cells - cytology
Endothelial Cells - physiology
Equipment Design
Fluoresceins
Fluorescent Dyes
HeLa Cells
Humans
Manipulation
Microfluidic Analytical Techniques
Mobility
Platforms
Reconstruction
Sound
Surface acoustic waves
Time-Lapse Imaging - methods
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Summary:Precise reconstruction of heterotypic cell–cell interactions in vitro requires the coculture of different cell types in a highly controlled manner. In this article, we report a standing surface acoustic wave (SSAW)-based cell coculture platform. In our approach, different types of cells are patterned sequentially in the SSAW field to form an organized cell coculture. To validate our platform, we demonstrate a coculture of epithelial cancer cells and endothelial cells. Real-time monitoring of cell migration dynamics reveals increased cancer cell mobility when cancer cells are cocultured with endothelial cells. Our SSAW-based cell coculture platform has the advantages of contactless cell manipulation, high biocompatibility, high controllability, simplicity, and minimal interference of the cellular microenvironment. The SSAW technique demonstrated here can be a valuable analytical tool for various biological studies involving heterotypic cell–cell interactions.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac502453z