A high-throughput cellulase screening system based on droplet microfluidics

A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we ha...

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Bibliographic Details
Published in:Biomicrofluidics 2014-07, Vol.8 (4), p.041102-041102
Main Authors: Ostafe, Raluca, Prodanovic, Radivoje, Lloyd Ung, W, Weitz, David A, Fischer, Rainer
Format: Article
Language:English
Subjects:
Cellulase
Fast Track
Fluorescence
Microfluidics
Screening
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Summary:A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells.
ISSN:1932-1058
1932-1058
DOI:10.1063/1.4886771