Nanoparticle Tension Probes Patterned at the Nanoscale: Impact of Integrin Clustering on Force Transmission

Herein we aimed to understand how nanoscale clustering of RGD ligands alters the mechano-regulation of their integrin receptors. We combined molecular tension fluorescence microscopy with block copolymer micelle nanolithography to fabricate substrates with arrays of precisely spaced probes that can...

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Bibliographic Details
Published in:Nano letters 2014-10, Vol.14 (10), p.5539-5546
Main Authors: Liu, Yang, Medda, Rebecca, Liu, Zheng, Galior, Kornelia, Yehl, Kevin, Spatz, Joachim P, Cavalcanti-Adam, Elisabetta Ada, Salaita, Khalid
Format: Article
Language:English
Subjects:
Adhesion
Animals
Cell Adhesion
Cell Line
Clustering
Condensed matter: structure, mechanical and thermal properties
Cross-disciplinary physics: materials science
rheology
Exact sciences and technology
Fibronectins - metabolism
Fluorescence
Focal Adhesions - metabolism
Focal Adhesions - ultrastructure
Gold - chemistry
Humans
Integrins - metabolism
Letter
Ligands
Low-dimensional structures (superlattices, quantum well structures, multilayers): structure, and nonelectronic properties
Materials science
Mechanotransduction, Cellular
Metal Nanoparticles - chemistry
Metal Nanoparticles - ultrastructure
Methods of nanofabrication
Mice
Microscopy
Microscopy, Fluorescence
Nanocrystalline materials
Nanolithography
Nanoscale materials and structures: fabrication and characterization
Nanostructure
NIH 3T3 Cells
Oligopeptides - chemistry
Oligopeptides - metabolism
Physics
Receptors
Strategy
Surfaces and interfaces
thin films and whiskers (structure and nonelectronic properties)
Tissue Array Analysis
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Description
Summary:Herein we aimed to understand how nanoscale clustering of RGD ligands alters the mechano-regulation of their integrin receptors. We combined molecular tension fluorescence microscopy with block copolymer micelle nanolithography to fabricate substrates with arrays of precisely spaced probes that can generate a 10-fold fluorescence response to pN-forces. We found that the mechanism of sensing ligand spacing is force-mediated. This strategy is broadly applicable to investigating receptor clustering and its role in mechanotransduction pathways.
ISSN:1530-6984
1530-6992
DOI:10.1021/nl501912g