Growth promoting effects of some lichen metabolites on probiotic bacteria

• Published in: Journal of food science and technology 2014-10, Vol.51 (10), p.2624-2631
• Main Authors: Gaikwad, Subhash, Verma, Neeraj, Sharma, B. O., Behera, B. C.
• Format: Article
• Language: English
• Subjects: Acids; Antioxidants; Bacteria; Biomass; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Dry matter; Everniastrum; Food products; Food Science; Functional foods & nutraceuticals; Lactobacillus casei; Lichens; Lipids; Metabolites; Nutrition; Original; Original Article; Phytochemicals; Probiotics; Proteins; Secondary metabolites; Solvents; Studies
• ISSN: 0022-1155; 0975-8402
• DOI: 10.1007/s13197-012-0785-x

In the present study, the extract of four natural lichen species Canoparmelia eruptens , Everniastrum cirrhatum , Parmotrema austrosinense and Rimelia cetrata were studied for the source of natural antioxidant and their purified secondary metabolites were evaluated for growth promoting effects on probiotic bacteria Lactobacillus casei . The methanolic fraction of lichen species showed moderate to high antioxidant activity in the order P. austrosinense > E. cirrhatum > C. eruptens > R. cetrata. The lichen metabolites showed antioxidant activity with an IC 50 values (μg/ml); lecanoric acid 79–95, salazinic 88–108, atranorin 100–116 and consalazinic acid 119–125. As far as the growth promoting effects of lichen metabolites on L. casei is concerned, lecanoric acid at 100 μg/ml conc. showed high growth stimulating activity in terms of increased dry matter of biomass (56.08 mg) of L. casei . Other lichen metabolites; salazinic acid, atranorin and consalazinic acid produced relatively less dry biomass 43.98 mg, 41.1 mg, 40.68 mg, respectively. However, standard antioxidants butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and Trolox after 36 h produced 39.04–47.81 mg dry biomass. At lower pH the growth promoting activity of lichen metabolites was found stable.