The E. coli single protein production system for production and structural analysis of membrane proteins

At present, only 0.9% of PDB-deposited structures are of membrane proteins in spite of the fact that membrane proteins constitute approximately 30% of total proteins in most genomes from bacteria to humans. Here we address some of the major bottlenecks in the structural studies of membrane proteins...

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Bibliographic Details
Published in:Journal of structural and functional genomics 2010-03, Vol.11 (1), p.81-84
Main Authors: Mao, Lili, Vaiphei, S. Thangminlal, Shimazu, Tsutomu, Schneider, William M., Tang, Yuefeng, Mani, Rajeswari, Roth, Monica J., Montelione, Gaetano T., Inouye, Masayori
Format: Article
Language:English
Subjects:
Amino acids
Biochemistry
Bioinformatics
Biomedical and Life Sciences
Cell growth
Chromatography
Cloning
E coli
Escherichia coli
Forestry Management
Genomes
Genomics
Human Genetics
Humans
Life Sciences
Medical research
Membrane Proteins - chemistry
Membrane Proteins - genetics
Membrane Proteins - metabolism
Microbial Genetics and Genomics
Molecular biology
Plant Genetics and Genomics
Protein synthesis
Proteins
Proteins - chemistry
Proteins - genetics
Proteins - metabolism
Signal transduction
Toxins
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Summary:At present, only 0.9% of PDB-deposited structures are of membrane proteins in spite of the fact that membrane proteins constitute approximately 30% of total proteins in most genomes from bacteria to humans. Here we address some of the major bottlenecks in the structural studies of membrane proteins and discuss the ability of the new technology, the Single-Protein Production system, to help solve these bottlenecks.
ISSN:1345-711X
1570-0267
DOI:10.1007/s10969-009-9070-2