Biotic elicitor enhanced production of psoralen in suspension cultures of Psoralea corylifolia L

• Published in: Saudi journal of biological sciences 2014-11, Vol.21 (5), p.499-504
• Main Authors: Ahmed, Syed Abrar, Baig, Mirza Mushtaq Vaseem
• Format: Article
• Language: English
• Subjects: Aspergillus niger; callus; cell culture; chitosan; Cullen corylifolium; cultured cells; Elicitation; elicitors; fungi; leaves; Original; Penicillium chrysogenum; Plant cell culture; Psoralea corylifolia L; Psoralen; Psoralens; scotophase; Suspension cultures; yeast extract
• ISSN: 1319-562X; 2213-7106
• DOI: 10.1016/j.sjbs.2013.12.008

Cell cultures of Psoralea corylifolia L. were established from the leaf disk derived callus. The effect of different biotic elicitors prepared from the fungal extract (Aspergillus niger and Penicillium notatum), yeast extract and chitosan with different concentrations was studied. The increased synthesis of psoralen in 16-day old cell cultures under 16h of light and 8h of dark period was studied. Elicitation of psoralen in A. niger elicitor treated cells was found 9-fold higher over control cells. Treating the cells with P. notatum, yeast extract and chitosan elicitors lead to four to seven-fold higher psoralen accumulation over control cells. The extract of A. niger at 1.0% v/v increased the significant accumulation of psoralen (9850μg/g DCW) in the cultured cells. Our study clearly shows that all the elicitors had the potential to increase the accumulation of psoralen but the A. niger elicitor at 1.0% v/v induced maximum accumulation.