Selection of Functional Human Sperm with Higher DNA Integrity and Fewer Reactive Oxygen Species

Fertilization and reproduction are central to the survival and propagation of a species. Couples who cannot reproduce naturally have to undergo in vitro clinical procedures. An integral part of these clinical procedures includes isolation of healthy sperm from raw semen. Existing sperm sorting metho...

Full description

Saved in:
Bibliographic Details
Published in:Advanced healthcare materials 2014-10, Vol.3 (10), p.1671-1679
Main Authors: Asghar, Waseem, Velasco, Vanessa, Kingsley, James L., Shoukat, Muhammad S., Shafiee, Hadi, Anchan, Raymond M., Mutter, George L., Tüzel, Erkan, Demirci, Utkan
Format: Article
Language:English
Subjects:
advance reproductive technologies
Assaying
Biological and medical sciences
Cell Separation - methods
Chips
Couples
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA - metabolism
DNA Fragmentation
DNA integrity
Fragmentation
Humans
Integrity
Male
Medical sciences
Operators
Oxygen
reactive oxygen species
Reactive Oxygen Species - analysis
Reactive Oxygen Species - metabolism
reproduction
Reproductive Techniques, Assisted
Sorting
Sperm
sperm sorting
Spermatozoa - cytology
Spermatozoa - metabolism
Spermatozoa - physiology
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Technology. Biomaterials. Equipments
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fertilization and reproduction are central to the survival and propagation of a species. Couples who cannot reproduce naturally have to undergo in vitro clinical procedures. An integral part of these clinical procedures includes isolation of healthy sperm from raw semen. Existing sperm sorting methods are not efficient and isolate sperm having high DNA fragmentation and reactive oxygen species (ROS), and suffer from multiple manual steps and variations between operators. Inspired by in vivo natural sperm sorting mechanisms where vaginal mucus becomes less viscous to form microchannels to guide sperm towards egg, a chip is presented that efficiently sorts healthy, motile and morphologically normal sperm without centrifugation. Higher percentage of sorted sperm show significantly lesser ROS and DNA fragmentation than the conventional swim‐up method. The presented chip is an easy‐to‐use high‐throughput sperm sorter that provides standardized sperm sorting assay with less reliance on operators's skills, facilitating reliable operational steps. Conventional sperm sorting methods employed in reproductive clinics to treat infertile couples involve centrifugation steps that damage sperm morphology and produce reactive oxygen species (ROS) and DNA fragmentation. Here, a centrifugation‐free high‐throughput sperm sorting assay that rapidly isolates functional sperm with high DNA integrity and fewer ROS is presented.
ISSN:2192-2640
2192-2659
DOI:10.1002/adhm.201400058