Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon

To develop stem cell therapy for small intestinal (SI) diseases, it is essential to determine whether SI stem cells in culture retain their tissue regeneration capabilities. By using a heterotopic transplantation approach, we show that cultured murine SI epithelial organoids are able to reconstitute...

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Bibliographic Details
Published in:Genes & development 2014-08, Vol.28 (16), p.1752-1757
Main Authors: Fukuda, Masayoshi, Mizutani, Tomohiro, Mochizuki, Wakana, Matsumoto, Taichi, Nozaki, Kengo, Sakamaki, Yuriko, Ichinose, Shizuko, Okada, Yukinori, Tanaka, Toshihiro, Watanabe, Mamoru, Nakamura, Tetsuya
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Colon - cytology
Colon - metabolism
Epithelial Cells - cytology
Epithelial Cells - transplantation
Epithelium - metabolism
Epithelium - ultrastructure
Intestine, Small - cytology
Intestine, Small - metabolism
Mice, Inbred C57BL
Models, Animal
Organoids - cytology
Paneth Cells - cytology
Paneth Cells - metabolism
Research Communication
Stem Cells - cytology
Stem Cells - metabolism
Transcriptome
Transplantation, Heterotopic
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Summary:To develop stem cell therapy for small intestinal (SI) diseases, it is essential to determine whether SI stem cells in culture retain their tissue regeneration capabilities. By using a heterotopic transplantation approach, we show that cultured murine SI epithelial organoids are able to reconstitute self-renewing epithelia in the colon. When stably integrated, the SI-derived grafts show many features unique only to the SI but distinct from the colonic epithelium. Our study provides evidence that cultured adult SI stem cells could be a source for cell therapy of intestinal diseases, maintaining their identity along the gastrointestinal tract through an epithelium-intrinsic mechanism.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.245233.114