Application the mass spectrometry MALDI-TOF technique for detection of Babesia canis canis infection in dogs

The aim of this study was to use rapid mass spectrometry (MS)-based proteomics analyses for diagnosis of Babesia canis canis infections in dogs. The study was conducted on two groups of dogs—healthy dogs and dogs infected with B. canis canis which demonstrated symptoms of babesiosis. The matrix-assi...

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Bibliographic Details
Published in:Parasitology research (1987) 2014-11, Vol.113 (11), p.4293-4295
Main Authors: Adaszek, Łukasz, Banach, Tomasz, Bartnicki, Michał, Winiarczyk, Dagmara, Łyp, Paweł, Winiarczyk, Stanisław
Format: Article
Language:English
Subjects:
Animals
Babesia
Babesia - isolation & purification
Babesia canis
babesiosis
Babesiosis - diagnosis
Biomarkers - blood
Biomedical and Life Sciences
Biomedicine
blood serum
Canis
desorption
Diseases
dog diseases
Dog Diseases - diagnosis
Dog Diseases - parasitology
Dogs
Female
Health aspects
Immunology
ionization
Male
matrix-assisted laser desorption-ionization mass spectrometry
Medical Microbiology
Methods
Microbiology
proteins
Proteomics
Sensitivity and Specificity
Short Communication
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Time-of-flight mass spectrometry
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Summary:The aim of this study was to use rapid mass spectrometry (MS)-based proteomics analyses for diagnosis of Babesia canis canis infections in dogs. The study was conducted on two groups of dogs—healthy dogs and dogs infected with B. canis canis which demonstrated symptoms of babesiosis. The matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS technique revealed the presence of a protein fraction of 51–52 kDa in the blood serum of all the animals infected with the protozoa, which was not found in the serum of healthy dogs. The proteins are suspected to be disease markers, whereas the MALDI-TOF technique itself has high specificity and sensitivity and can be applied in analytical laboratories in the diagnosis of canine babesiosis.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-014-4124-1