Evaluation of the Role of G Protein-Coupled Receptor Kinase 3 in Desensitization of Mouse Odorant Receptors in a Mammalian Cell Line and in Olfactory Sensory Neurons

Thousands of odors are sensed and discriminated by G protein-coupled odorant receptors (ORs) expressed in olfactory sensory neurons (OSNs). G protein-coupled receptor kinases (GRKs) may have a role in desensitization of ORs. However, whether ORs are susceptible to agonist-dependent desensitization a...

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Bibliographic Details
Published in:Chemical senses 2014-11, Vol.39 (9), p.771-780
Main Authors: KATO, Aya, REISERT, Johannes, IHARA, Sayoko, YOSHIKAWA, Keiichi, TOUHARA, Kazushige
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Calcium - analysis
Calcium - metabolism
Eugenol - metabolism
Fundamental and applied biological sciences. Psychology
G-Protein-Coupled Receptor Kinase 3 - metabolism
HEK293 Cells
Humans
Mice
Olfaction. Taste
Original
Perception
Psychology. Psychoanalysis. Psychiatry
Psychology. Psychophysiology
Receptors, Odorant - metabolism
Sensory Receptor Cells - metabolism
Smell
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Summary:Thousands of odors are sensed and discriminated by G protein-coupled odorant receptors (ORs) expressed in olfactory sensory neurons (OSNs). G protein-coupled receptor kinases (GRKs) may have a role in desensitization of ORs. However, whether ORs are susceptible to agonist-dependent desensitization and whether GRKs affect odorant responsiveness of OSNs are currently unknown. Here we show that GRK3 attenuated the agonist responsiveness of a specific mouse odorant receptor for eugenol (mOR-EG) upon agonist pretreatment in HEK293 cells, but GRK3 did not affect the response amplitude or the recovery kinetics upon repeated agonist stimulation. We performed electrophysiological recordings of single OSNs which expressed mOR-EG and green fluorescent protein (GFP) in the presence or absence of GRK3. The kinetics and amplitude of agonist responsiveness of individual GFP-labeled mOR-EG neurons were not significantly affected by the absence of GRK3. These results indicate that the role of GRK3 in attenuating ORs responsiveness in OSNs may have been overestimated.
ISSN:0379-864X
1464-3553
DOI:10.1093/chemse/bju050