Diversity of Bacteria Isolated from Antarctic Freshwater Reservoirs Possessing the Ability to Produce Polyhydroxyalkanoates

The diversity of polyhydroxyalkanoates-producing bacteria in freshwater reservoirs in the Ecology Glacier foreland, Antarctica, was examined by a cultivation-dependent method. Isolated strains were analyzed phylogenetically by 16S rRNA gene sequencing, and classified as members of Alpha-, Beta-, or...

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Bibliographic Details
Published in:Current microbiology 2014-11, Vol.69 (5), p.594-603
Main Authors: Ciesielski, Slawomir, Górniak, Dorota, Możejko, Justyna, Świątecki, Aleksander, Grzesiak, Jakub, Zdanowski, Marek
Format: Article
Language:English
Subjects:
Antarctic Regions
Bacteria
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Bacteria - metabolism
Biodiversity
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Cluster Analysis
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Fresh water
Fresh Water - microbiology
freshwater
Freshwater ecology
genes
Genes, Bacterial
Genetic diversity
Glaciers
Janthinobacterium
Life Sciences
Microbiology
Molecular Sequence Data
Phylogenetics
Phylogeny
Polyesters
Polyhydroxyalkanoates
Polyhydroxyalkanoates - metabolism
Polymerase Chain Reaction
Pseudomonas
Reservoirs
ribosomal RNA
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
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Summary:The diversity of polyhydroxyalkanoates-producing bacteria in freshwater reservoirs in the Ecology Glacier foreland, Antarctica, was examined by a cultivation-dependent method. Isolated strains were analyzed phylogenetically by 16S rRNA gene sequencing, and classified as members of Alpha-, Beta-, or Gammaproteobacteria classes. Polymerase chain reaction was used to detect PHA synthase genes. Potential polyhydroxyalkanoates (PHAs) producers belonging mainly to Pseudomonas sp., and Janthinobacterium sp. were isolated from all five sampling sites, suggesting that PHA synthesis is a common bacterial feature at pioneer sites. All Pseudomonas strains had the genetic potential to synthesize medium-chain-length PHAs, whereas some isolated Janthinobacterium strains might produce short-chain-length PHAs or medium-chain-length PHAs. It is the first report revealing that Janthinobacterium species could have the potential to produce medium-chain-length PHAs.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-014-0629-1