PROPOLIS AMELIORATES TUMOR NEROSIS FACTOR-α, NITRIC OXIDE LEVELS, CASPASE-3 AND NITRIC OXIDE SYNTHASE ACTIVITIES IN KAINIC ACID MEDIATED EXCITOTOXICITY IN RAT BRAIN

Background: Increased nitric oxide (NO), neuronal inflammation and apoptosis have been proposed to be involved in excitotoxicity plays a part in many neurodegenerative diseases. To understand the neuro-protective effects of propolis, activities of Nitric oxide synthase (NOS) and caspase-3 along with...

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Published in:African journal of traditional, complementary, and alternative medicines complementary, and alternative medicines, 2014-01, Vol.11 (5), p.48-53
Main Authors: Swamy, Mummedy, Suhaili, Dian, Sirajudeen, K. N. S, Mustapha, Zulkarnain, Govindasamy, Chandran
Format: Article
Language:English
Subjects:
Animals
Apitherapy
Apoptosis
Brain - drug effects
Brain - metabolism
Caspase 3 - metabolism
Caspase-3
Dietary Supplements
Excitotoxicity
Kainic Acid - toxicity
Male
Neurodegenerative Diseases - metabolism
Neurodegenerative Diseases - prevention & control
Neuroprotective Agents - pharmacology
Neurotoxicity Syndromes - metabolism
Neurotoxicity Syndromes - prevention & control
Nitric oxide
Nitric Oxide - metabolism
Nitric Oxide Synthase - metabolism
Propolis
Propolis - pharmacology
Rat Brain
Rats, Sprague-Dawley
Research Papers
TNF-α
Tumor Necrosis Factor-alpha - metabolism
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Summary:Background: Increased nitric oxide (NO), neuronal inflammation and apoptosis have been proposed to be involved in excitotoxicity plays a part in many neurodegenerative diseases. To understand the neuro-protective effects of propolis, activities of Nitric oxide synthase (NOS) and caspase-3 along with NO and tumor necrosis factor-α (TNF-α) levels were studied in cerebral cortex (CC), cerebellum (CB) and brain stem (BS) in rats supplemented with propolis prior to excitotoxic injury with kainic acid (KA). Materials and methods: Male Sprague-Dawley rats were divided into four groups (n=6 rats per group) as Control, KA, Propolis and KA+Propolis. The control group and KA group have received vehicle and saline. Propolis group and propolis + KA group were orally administered with propolis (150mg/kg body weight), five times every 12 hours. KA group and propolis +KA group were injected subcutaneously with kainic acid (15mg/kg body weight) and were sacrificed after 2 hrs. CC, CB and BS were separated, homogenized and used for estimation of NOS, caspase-3, NO and TNF-α by commercial kits. Results were analyzed by one way ANOVA, reported as mean + SD (n=6 rats), and p
ISSN:0189-6016
2505-0044
0189-6016
DOI:10.4314/ajtcam.v11i5.8