RESTORATION OF GLUTAMINE SYNTHETASE ACTIVITY, NITRIC OXIDE LEVELS AND AMELIORATION OF OXIDATIVE STRESS BY PROPOLIS IN KAINIC ACID MEDIATED EXCITOTOXICITY

Background: Propolis has been proposed to be protective on neurodegenerative disorders. To understand the neuroprotective effects of honeybee propolis, glutamine synthetase (GS) activity, nitric oxide (NO), thiobarbituric acid reactive substances (TBARS) and total antioxidant status (TAS) were studi...

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Bibliographic Details
Published in:African journal of traditional, complementary, and alternative medicines complementary, and alternative medicines, 2014-01, Vol.11 (2), p.458-463
Main Authors: Swamy, Mummedy, Norlina, Wan, Azman, Wan, Suhaili, Dian, Sirajudeen, K.N.S, Mustapha, Zulkarnain, Govindasamy, Chandran
Format: Article
Language:English
Subjects:
Animals
Brain - drug effects
Brain - enzymology
Brain - metabolism
Excitotoxicity
Glutamate-Ammonia Ligase - metabolism
Glutamine Synthetase
Kainic Acid - toxicity
Male
Neuroprotective Agents - pharmacology
Nitric oxide
Nitric Oxide - metabolism
Oxidative Stress
Oxidative Stress - drug effects
Propolis
Propolis - pharmacology
Rat Brain
Rats
Rats, Sprague-Dawley
Research Papers
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Summary:Background: Propolis has been proposed to be protective on neurodegenerative disorders. To understand the neuroprotective effects of honeybee propolis, glutamine synthetase (GS) activity, nitric oxide (NO), thiobarbituric acid reactive substances (TBARS) and total antioxidant status (TAS) were studied in different brain regions- cerebral cortex (CC), cerebellum (CB) and brain stem (BS) of rats supplemented with propolis and subjected to kainic acid (KA) mediated excitotoxicity. Materials and Methods: Male Sprague-Dawley rats were divided into four groups; Control group and KA group received vehicle and saline. Propolis group and propolis + KA group were orally administered with propolis (150mg/kg body weight), five times every 12 hours. KA group and propolis + KA group were injected subcutaneously with kainic acid (15mg/kg body weight) and were sacrificed after 2 hrs and CC, CB and BS were separated homogenized and used for estimation of GS activity, NO, TBARS, and TAS concentrations by colorimetric methods. Results were analyzed by oneway ANOVA, reported as mean ± SD from 6 animals, and p
ISSN:0189-6016
2505-0044
0189-6016
DOI:10.4314/ajtcam.v11i2.33