Molecular mechanisms of paclitaxel and NM-3 on human gastric cancer in a severe combined immune deficiency mice orthotopic implantation model

To explore the molecular mechanisms of action of paclitaxel and NM-3 on human gastric cancer in severe combined immune deficiency (SCID) mice. Human gastric cancer cells SGC-7901 were implanted into SCID mice and mice were treated with paclitaxel and NM-3. The effects of paclitaxel and NM-3 on apopt...

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Bibliographic Details
Published in:World journal of gastroenterology : WJG 2007-08, Vol.13 (30), p.4131-4135
Main Authors: Zhu, Jin-Shui, Song, Ming-Quan, Chen, Guo-Qiang, Li, Qin, Sun, Qun, Zhang, Qiang
Format: Article
Language:English
Subjects:
Adenocarcinoma - drug therapy
Adenocarcinoma - pathology
Animals
Antigens, Viral, Tumor - drug effects
Antigens, Viral, Tumor - genetics
Antineoplastic Agents - pharmacology
Apoptosis - drug effects
Cell Line, Tumor
Disease Models, Animal
Drug Synergism
Drug Therapy, Combination
Humans
Isocoumarins - pharmacology
Male
Mice
Mice, Nude
Mice, SCID
Paclitaxel - pharmacology
Rapid Communication
Stomach Neoplasms - drug therapy
Stomach Neoplasms - pathology
Viral Core Proteins - drug effects
Viral Core Proteins - genetics
Xenograft Model Antitumor Assays
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Summary:To explore the molecular mechanisms of action of paclitaxel and NM-3 on human gastric cancer in severe combined immune deficiency (SCID) mice. Human gastric cancer cells SGC-7901 were implanted into SCID mice and mice were treated with paclitaxel and NM-3. The effects of paclitaxel and NM-3 on apoptosis of human gastric cancer cells were analyzed using flow cytometry, TUNEL assays, and DNA fragment analyses. Apoptosis of SGC-7901 cells was successfully induced by paclitaxel, NM-3, and the combination of paclitaxel and NM-3 24 h after injection as shown by the presence of apoptotic hypodiploid peaks on the flow cytometer before G1-S and a characteristic apoptotic band pattern in the DNA electrophoresis. The apoptotic rate detected by TUNEL assay was found to be significantly higher in the paclitaxel/NM-3 compared to the control group (38.5% +/- 5.14% vs 13.2% +/- 1.75%, P < 0.01). Paclitaxel in combination with NM-3 is able to induce apoptosis of the human gastric cancer cells in SCID mice effectively and synergistically.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v13.i30.4131