HIF-1α regulated tongue squamous cell carcinoma cell growth via regulating VEGF expression in a xenograft model

We aimed to study the mechanism of hypoxia-inducible factor 1 alpha (HIF-1α) regulating the cell proliferation of tongue squamous cell carcinoma (TSCC) via vascular endothelial growth factor (VEGF). We used RNA interference (RNAi) technique, transfected chemically synthesized siRNA against HIF-1α in...

Full description

Saved in:
Bibliographic Details
Published in:Annals of translational medicine 2014-09, Vol.2 (9), p.92-92
Main Authors: Liang, Jun, Zhang, Zhaoqiang, Liang, Lizhong, Shen, Yun, Ouyang, Kexiong
Format: Article
Language:English
Subjects:
Original
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We aimed to study the mechanism of hypoxia-inducible factor 1 alpha (HIF-1α) regulating the cell proliferation of tongue squamous cell carcinoma (TSCC) via vascular endothelial growth factor (VEGF). We used RNA interference (RNAi) technique, transfected chemically synthesized siRNA against HIF-1α into CAL-27 cells, and detected the expression of HIF-1α and VEGF by real time-PCR and Western blotting in order to find out if HIF-1α regulated the expression of VEGF. A xenograft experiment was carried out to observe the role of HIF-1α on the tumor growth of tongue squamous cell carcinoma. HIF-1α and VEGF mRNA expression was significantly downregulated 36 and 48 h after transfection (P
ISSN:2305-5839
2305-5839
DOI:10.3978/j.issn.2305-5839.2014.08.01