N-Methyl-4-hydrazino-7-nitrobenzofurazan: a fluorogenic substrate for peroxidase-like DNAzyme, and its potential application

Characterization and optimization studies of N -methyl-4-hydrazino-7-nitrobenzofurazan (MNBDH) as a new fluorogenic substrate in the peroxidation reaction catalyzed by DNAzyme are reported. The effects of pH, H 2 O 2 concentration, metal-cation type, and the concentration and type of surfactant on t...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2014, Vol.406 (28), p.7049-7057
Main Authors: Kosman, Joanna, Wu, Yu-Tang, Gluszynska, Agata, Juskowiak, Bernard
Format: Article
Language:English
Subjects:
Analysis
Analytical Chemistry
Assaying
Benzene
Biochemistry
Biosensing Techniques
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Deoxyribonucleic acid
DNA
DNA, Catalytic - metabolism
DNA, Single-Stranded - analysis
DNA, Single-Stranded - chemistry
Electric properties
Enzymes
Experiments
Fluorescence
Fluorescent Dyes - chemistry
Food Science
Hydrazines - metabolism
Hydrogen Peroxide
Laboratory Medicine
Limit of Detection
Mathematical analysis
Metal concentrations
Metal ions
Monitoring/Environmental Analysis
Optimization
Oxadiazoles - metabolism
Oxidation
Paper in Forefront
Peroxidase
Peroxidases - metabolism
Peroxidation
Peroxides
Single-stranded DNA
Substrates
Surfactants
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Summary:Characterization and optimization studies of N -methyl-4-hydrazino-7-nitrobenzofurazan (MNBDH) as a new fluorogenic substrate in the peroxidation reaction catalyzed by DNAzyme are reported. The effects of pH, H 2 O 2 concentration, metal-cation type, and the concentration and type of surfactant on the fluorescence intensity were investigated. The optimized reaction was subsequently used for the development of an assay for DNA detection based on a molecular-beacon probe. The use of a fluorogenic substrate enabled the detection of a single-stranded DNA target with a 1 nmol L −1 detection limit. Graphical Abstract ᅟ
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-014-8119-7